DNA purification Flashcards
1
Q
add washing up liquid
A
degrade cell membrane
2
Q
add sodium chloride
A
shield negatively charged phosphate groups of the DNA molecules causing them to coalesce
3
Q
place in 60c water bath for 15 min
A
partially denature the DNAse enzyme
4
Q
place in ice bath for 5 min
A
slow breakdown of DNA
5
Q
blend for 5 s
A
degrade cell walls and membrans further, permitting release of DNA
6
Q
filter
A
obtain filtrate containing proteins and DNA
7
Q
add protease
A
to hydrolyse proteins associated with DNA
8
Q
add ice cold ethanol
A
to precipitate DNA as it is insoluble in ethanol
