dna mixtures Flashcards
1
Q
what is a stutter
A
a natural product of the PCR
Usually 2 represent unit less than parent allele
Also observed at -8bp and +4bp
2
Q
What are stutter thresholds
A
measured as a percentage of parent allele height
3
Q
what is the peak height ratio for heterozygous loci
A
paired alleles are not equally amplified
stochastic amplification of one allele during PCR
preferential application of shorter allele during PCR
presence of PCR inhibitor
primer binding site mutation decreasing binding efficiency
degradation of shorter allele
