DNA manipulation Flashcards
What is DNA manipulation?
The process of altering the genetic material of an organism.
True or False: DNA manipulation can only occur in bacteria.
False
Fill in the blank: The technique used to cut DNA at specific sequences is called ______.
restriction enzyme digestion
What is the purpose of PCR (Polymerase Chain Reaction)?
To amplify small segments of DNA.
Which of the following is a common application of DNA manipulation? (a) Gene therapy (b) Cloning (c) Genetic modification of crops (d) All of the above
d) All of the above
What role do plasmids play in DNA manipulation?
They serve as vectors to transfer genetic material into cells.
True or False: CRISPR is a technology used for genome editing.
True
What does the acronym CRISPR stand for?
Clustered Regularly Interspaced Short Palindromic Repeats
Fill in the blank: The process of introducing foreign DNA into a cell is known as ______.
transfection
What is a transgenic organism?
An organism that has been genetically modified to contain DNA from another species.
Which enzyme is commonly used to join DNA fragments together?
DNA ligase
What is the purpose of gel electrophoresis in DNA manipulation?
To separate DNA fragments based on size.
True or False: Gene editing can only be performed on somatic cells.
False
What is the main advantage of using CRISPR over traditional gene editing methods?
It allows for more precise and efficient modifications.
What is a gene drive?
A genetic engineering technology that increases the likelihood of a specific trait being passed on to the next generation.
Fill in the blank: The sequence of nucleotides in DNA is known as its ______.
genetic code
What is the function of a promoter in DNA?
It initiates the transcription of a gene.
True or False: All DNA manipulation techniques are ethical.
False
What is the purpose of a selectable marker in cloning?
To identify cells that have successfully taken up the foreign DNA.
What are homologous recombination techniques used for?
To replace or edit specific genes in an organism’s genome.
Which of the following is NOT a method of DNA manipulation? (a) Electroporation (b) Microinjection (c) Photosynthesis (d) Lipofection
c) Photosynthesis
What is the primary ethical concern regarding DNA manipulation?
Potential unintended consequences and long-term effects on ecosystems and human health.
True or False: DNA sequencing is a part of DNA manipulation.
True
