DNA II Flashcards
Haploid
Half the # of chromosomes
Heterozygous
2 diff alleles for a given gene
Genotype
The genetic makeup/ set of alleles of an organism
DNA fingerprint
Used to link criminals to evidence found at the crime scene
Allelic Ladder
Used to compare the samples to
5 Possible Lab Mistakes
- Not changing tips
- Not wearing gloves
- Didn’t vortex it
- Tipped micropipetter
Falsifiable
Capacity for some proposition, statement, theory of hypothesis to be proven wrong
Parsimony
The principle of choosing the simplest scientific explanation to fit the evidence
Variable
An element, feature, or factor that is liable to vary or change
Control
A variable which remains unchanged or held constant
Replication
Using more that one group of subjects for each treatment.
Dependant Variable
AKA response variable. Measuring as a response to the independent variable.
Independant Variable
AKA treatment variable.
The one we are manipulating or monitoring
Error bars
Represent the uncertainty, error of the corresponding coordinate of the point
3 conditions for Hypotheses
- Has to be falsifiable
- Consider rule of parsimony
- Should have the potential to
explain future phenomena
Gene
A piece of DNA, discrete unit of hereditary information consisting of a specific nucleotide sequence in DNA
Transcription
Synthesis of RNA using a DNA template
RNA Polymerase
AN enzyme which links ribonucleotides into a growing RNA chain during transcription
mRNA
Attaches to the ribosomes in the cytoplasm and specifies the primary structure of a protein
Translation
Synthesis of a polypeptide using the genetic information encoded in an mRNA molecule
Codon
3 nucleotide sequence of DNA or mRNA that specifies a particular amino acid or termination signal
Reading Frame
On an mRNA, the triplet grouping of ribonucleotides used by the translation machinery during polypeptide synthesis
Secondary Structure
Regions of repetitive coiling or folding of the polypeptide backbone of a protein due to H-Bonding between constituents of the backbone
Assay as a NOUN
The test of concentration
Assay as a VERB
Determining an unknown concentration from a standard curve
Chromophore
An atom or group whose presence is responsible for the colour of a compound