DNA, genes and protein synthesis Flashcards

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1
Q

How is prokaryotic DNA different to that of eukaryotic

A

it is short

it is circular

it is not bound by proteins

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2
Q

Eukaryotic DNA structure

A

Long

Linear

Bound with histone proteins

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3
Q

Mitochondria and chloroplast DNA

A

Short, circular and not bound with histones

similar to prokaryotic DNA

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4
Q

What is a gene

A

a short base sequence of DNA that codes for the amino acid sequence of a polypeptide or functional RNA (rRNA + tRNA)

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5
Q

Where are genes found

A

they occupy fixed positions on a DNA strand called a locus

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6
Q

What is a codon

A

a triplet of bases that encode a specific amino acid

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7
Q

3 properties of genetic code and what do they mean

A

its universal ( the code is the same for all organisms )

its degenerate ( there are more codons than amino acids, so some codons code for the same amino acid )

Non overlapping ( each base is only part of one codon so all codons are a discrete unit )

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8
Q

Difference between introns and exons

A

coding parts of DNA are exons

non coding parts of DNA are introns

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9
Q

What is a genome

A

the complete set of genes in a cell

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10
Q

What is the proteome

A

full range of proteins that a cell can produce from the genome

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11
Q

What does transcription do

A

Converts DNA into pre-mRNA/mRNA

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12
Q

What is splicing and what cells does it occur in

A

Conversion of pre-mRNA to mRNA in eukaryotic cells

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13
Q

What is splicing and what cells does it occur in

A

Conversion of pre-mRNA to mRNA in eukaryotic cells

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14
Q

What is translation

A

Conversion of mRNA to proteins using tRNA

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15
Q

Structure of mRNA

A

Short, single stranded linear chain of ribonucleotides

each 3 adjacent bases are called codons

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16
Q

Structure of tRNA

A

Single polynucleotide strand with a clover shape

has an amino acid attachment site and an anticodon complementary to an mRNA codon

17
Q

Stages of transcription

A

DNA helicase unwinds the two DNA strands by breaking hydrogen bonds between bases

Free RNA nucleotides align and pair with their complementary base

RNA polymerase joins mRNA nucleotides together to form pre-mRNA

18
Q

Stages of splicing

A

Introns are removed

exons are attached back together by a condensation reaction

mRNA is formed which exits through nuclear pores into cytoplasm

19
Q

Stages of translation

A

Ribosome attaches to start codon of mRNA
( AUG)

tRNA with complementary anticodon aligns with mRNA

ribosome moves along mRNA allowing tRNA to attach the the next codon along

the two amino acids attached to adjacent tRNA molecules are attached by peptide bonds

process is continued until ribosome reaches a stop codon, the ribosome then detaches and translation stops