DNA, Genes And Chromosomes Flashcards
Nuclear eukaryotic DNA is ____________
Linear
Describe the structure of DNA
- long, can be wound up by just ones
- double helix
- linear
How and why does prokaryotic DNA condense?
It supercoils could to fit within the cell
What does DNA contain?
Genes
What is a gene?
A sequence of DNA bases that code for polypeptide or functional RNA
What determines the order of amino acid within a polypeptide?
The order of bases
What sections make up DNA?
- exons and introns
What are exons?
Genes that do code for polypeptides
What are introns?
Non-coding genes within a sequence
What are alleles?
Genes that exist in more than one form
What are the two types of RNA?
mRNA- carries genetic code from DNA to Ribosomes
tRNA- used in translation, carries specific amino acids to ribosomes, it has a clover shape
What is the first stage of Protein Synthesis?
Transcription
Describe the process of protein synthesis
- RNA helicase attached to DNA helix at the beginning of a gene
- Hydrogen bonds between the two strands are hydrolysed separating the strand and uncoiling it
- One of the strands it’s then used as a template to make an mRNA copy
- RNA polymerase joins together free nucleotides to the base via complimentary base pairing, AU, CG
- RNA polymerase moves along the DNA separating the strands and assembling the pre-mRNA strand
- Hydrogen bonds between the uncoiled DNA reform and coil the DNA strands back into a double helix
- When RNA polymerase reaches a stop signal it detached from the DNA
- pre-mRNA is spliced and then moves out of the nucleus through the pore
What’s the second stage of Protein Synthesis?
Translation
Describe the process of Translation
- mRNA attaches to a ribosome and tRNA then carry the specific amino acids to it, ATP providing the energy needed
- A tRNA molecule with an anticodon that’s that’s complimentary to the first codon on the mRNA attaches to the mRNA via specific base pairing
- A second tRNA molecule attaches to the next codon on the mRNA the same way
- The two amino acids attached to the tRNA molecules are joined via a peptide bond
- A third tRNA molecule binds to the next codon on mRNA
- This process continues, forming a polypeptide chain
- The polypeptide chain moves away from the ribosome and translation is complete