DNA gel electrophoresis Flashcards
1
Q
Equipment (11)
A
- selected restriction enzymes
- agar gel
- gel tank
- electrical supply
- micropipettes
- DNA sample
- Loading dye
- UV light
- Camera
- Buffer solution
- DNA restriction ladder
2
Q
Outline the method
A
Mix DNA with desired restriction enzyme and loading dye. Prepare agar and pour into electrophoresis mould. Once set, fill electrophoresis tank with buffer solution. Use micropipette to load restriction ladder into first well then DNA samples cut with restriction enzyme into the other wells. Connect to electrical supply, turn on and leave until the dye has moved to the opposite end of the gel tank. Switch off and disconnect electrical supply. Carefully remove the gel from the tank and view under UV light. Take picture if desired.
