DNA fingerprinting

Question 1

what is genetic fingerprinting ?

Answer 1

the examination of VNTRS

Question 2

What is the probability of 2 individuals having the same VNTRs

Answer 2

Very low

Question 3

However the more closely related you are ?

Answer 3

The more similar the VNTRS are

Question 4

What is genetic fingerprinting ?

Answer 4

The analysis of VNTR DNS fragments

Question 5

What can genetic fingerprinting be used for ?

Answer 5

Used to determine genetic relationships and the genetic variability within a population

Question 6

Stages of genetic fingerprinting

Answer 6

Collection
Extraction
Digestion
Separation
hybridisation
Development
Analysis

Question 7

Collection

Answer 7

Smallest sample of dna can be collected for genetic fingerprinting
Could be from blood body cells or hair folllicles

Question 8

What happens if the dna sample is small?

Answer 8

Then PCR is used to amplify the amount of .dna

Question 9

Digestion

Answer 9

Restriction endonucleases are added to cut the dna into smaller fragments
Enzymes which cut close to the target VNTRs are added

Question 10

Separation gel electrophoresis

Answer 10

The dna sample,are loaded into smaller fragments Enzymes wells in agar gel
The gel is placed in a buffer liquid with an electrical voltage applied
D.NA is negatively charged so the dna samples OFE through the gel towards the positive end of the gel