Dna Fingerprinting

Question 1

What are small tandem repeats? Str markers o4 microsatellite dna

Answer 1

A unique set of genetic markers of an individual that makes its dna fingerprint and genetic profile and their presence or absence is determined mostly by PCR using specific primers for the marker

Question 2

Features of markers

Answer 2

Eukaryotic DNA contains some non coding sequences where two to six base pairs are repeated tandems (one after the other) 100 to 1000 times so that the lengths if these repats vary.

Since they r non coding variations do not have an impact in the phenotype

Question 3

Advantages of using Str markers

Answer 3

Occur frequently in the genome
Easily amplified by PCR
Highly variable polymorphisms
A large number of characterised STR r available

Question 4

What is used in dna profiling?

Answer 4

A set of markers(probes or pcr primers)

Question 5

Why can’t a dna fingerprint be obtained using one marker

Answer 5

There are many people with the same banding pattern

Question 6

What happens when more markers are used in combination

Answer 6

The probability of finding the same pattern is reduced

Question 7

What isbthe probabaitltity if 13 markers are used?

Answer 7

10 billion to several trillions

Question 8

Whynjs it highly unlikely that two people would have the same genetic profile/fingerprint.?

Answer 8

World population is 7 billion

Question 9

What are the applications of Dna fingerprinting

Answer 9

Criminal and victim identification
Paternity testing
Identification of infectious agents