DNA and proteins Flashcards
what parts of the gene are copied in transcription
both introns and exons
which direction is the:
coding strand
template strand
5’ - 3’
3’ - 5’
what are spliced out in eukaryotes
introns out exons join
where does transcription initiate
at the 5’ end, upstream of the promotor at the 5’ UTR
where does transcription terminate
at end of 3’ UTR
what does the mRNA look like/ contain
5’ cap - 5’ UTR - exons joined - 3’UTR - poly A tail
what is the translational start codon and the aa
AUG - methionine
what is the 5’ end of the protein named
the Amino end N terminus
what is the 3’ end of the protein named
the carboxyl end C terminus
what is the CDS
coding sequence - start to stop
What is the ORF
Open reading frame - start to stop but may include introns so different to CDS
what do exons contain
protein coding information
what does in silico mean
on computer
what can you use to predict the splicing at ATG
transcriptome data - mRNA and genome data - you can see where the first ATC/AUG is and where the stop codon is - work back to find introns/exons
what is cDNA
complementary DNA - synthesised - complementary to mRNA
