dna analysis Flashcards

1
Q

In 1901, he announced one of the most significant discoveries of the twentieth century—the typing of blood

A

Karl Landsteiner

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2
Q

It is a type of protein found on the outside of red blood cells. The protein is genetically inherited. If you have the protein, you are Rh-positive. If you did not inherit the protein, you are Rh-negative

A

Rh factor or Rhesus factor

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3
Q

In theory, no two individuals, except for identical twins, could be expected to have the same combination of blood factors. In other words, blood factors are controlled genetically and have the potential of being a highly distinctive feature for personal identification

A

True

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4
Q

The molecules that carry the body’s

genetic information

A

Deoxyribonucleic acid (DNA)

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5
Q

refers to a highly complex mixture of cells, enzymes, proteins, and inorganic substances

A

blood

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6
Q

It is the fluid portion of blood.

It is composed principally of water and accounts for 55 percent of blood content.

A

Plasma

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7
Q

The solid portion of blood is suspended in the plasma and accounts for 45 percent of its content.

A

red blood cells (i.e., erythrocytes), white blood cells (i.e., leukocytes), and platelets.

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8
Q

What happens when a protein in the plasma known as fibrin traps and enmeshes
the red blood cells?

A

Blood clot

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9
Q

If the clotted material were removed from the blood, a pale yellowish liquid known as ___ would be left.

It also contains proteins known as antibodies.

A

serum

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10
Q

They transport oxygen from the lungs to the body tissues and remove carbon dioxide from tissues by transporting it back to the lungs, where it is exhaled.

A

Red blood cells (RBC)

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11
Q

On the surface of each red blood cell are millions of characteristic chemical structures called?

A

Antigens

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12
Q

All type A individuals have A antigens.

A

True

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13
Q

All type B individuals have B antigens.

A

True

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14
Q

All AB individuals contain both A and

B antigens.

A

True

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15
Q

All type O individuals have neither A nor B antigens

on their cells.

A

True

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16
Q

Those people who have the D antigen
are said to be Rh positive; those without this antigen
are Rh negative.

A

True

17
Q

The fundamental principle of blood typing is that, for every antigen, there exists a specific antibody

A

True

18
Q

The antibody-containing serum is referred to as the ____, meaning a serum that reacts against something (i.e., antigens).

A

antiserum

19
Q

An antibody reacts only with its specific antigen
and no other. Thus, if serum containing anti-B is added
to red blood cells carrying the B antigen, the two will
combine, causing the antibody to attach itself to the
cell.. This creates a vast network of cross-linked cells usually seen in the form of clumping, or agglutination

A

True’

20
Q

Antibodies have two reactive sites. This means that each antibody can simultaneously be attached to antigens located on two different red blood cells.

A

Antibodies are bivalent

21
Q

A protein in the blood serum that
destroys or inactivates a specific
antigen.

A

antibody

22
Q

Blood serum that contains specific

antibodies.

A

antiserum

23
Q

The clumping together of red
blood cells by the action of an
antibody.

A

agglutination

24
Q

To produce antibodies capable of reacting with drugs, a specific drug is combined with a protein, and this combination is injected into an animal
such as a rabbit. This drug–protein complex acts as an antigen, stimulating the animal to produce antibodies. The recovered blood serum of the animal will now contain antibodies that are specific or nearly specific to the drug.

A

True

25
Q

It is designed as a urine dipstick test for blood, the strip can be moistened with distilled
water and placed in contact with a suspect bloodstain. The appearance of a green color indicates the presence of blood.

A

Hemastix strips

26
Q

After spraying ____ reagent onto
suspect items, agents darken the room; any bloodstains produce a faint blue
glow, known as luminescence.

A

luminol

27
Q

A relatively new product that is now available to be used in place of luminol.

A

Bluestar

28
Q

Luminol and Bluestar will not interfere

with any subsequent DNA testing

A

True

29
Q

Both depend on the addition of specific chemicals to the blood to form characteristic crystals
containing hemoglobin derivatives.

A

Takayama and Teichmann tests

the 2 are types of microcrystalline test?

30
Q

They are far less sensitive than color tests for blood identification and are more susceptible to interference
from contaminants that may be present in the stain

A

Crystal tests

31
Q

The standard test that determines
whether the blood is of human or animal origin.

It uses antisera, normally derived from rabbits that have been injected with the blood of a known animal, to determine the species origin of a questioned bloodstain.

A

Precipitin test

32
Q

Antibodies and antigens diffuse or move toward one another on a plate coated with a gel medium made from a natural polymer called agar. The
extracted bloodstain and the human antiserum are placed in separate holes opposite each other on the gel. If the blood is human, a line of precipitation
forms where the antigens and antibodies meet.

A

Gel diffusion

33
Q

An electrical potential is applied to the gel medium; a specific antigen–antibody reaction is denoted by a line of precipitation formed between the hole containing the blood extract and the hole containing the human antiserum

A

Electrophoretic method

34
Q

The determination that a substance is blood is best made by means of a preliminary color test. A positive result from the ______ is highly indicative of blood.

A

Kastle-Meyer color test