DNA Flashcards
Locus
Position of a gene on a strand of DNA.
Genes
are short sections of DNA that contain coded information as a specific sequence of bases. Genes code for polypeptides that determine the nature and development of organisms.
Mutation
A change in the base sequence of a gene
Alleles
alternative forms of a gene (created through mutations).
Codon
A sequence of three bases (called a triplet) that codes for a specific amino acid.
The base sequence of a gene determines the amino acid sequence in a polypeptide.
Exons
sequences of bases in a gene that code for the polypeptide
Introns:
(In eukaryotes), sequences of bases in a gene that do not code for polypeptides.
Differences in base sequences of alleles of a single gene may result in non-functional proteins, including non-functional enzymes.
Compare tRNA vs mRNA
tRNA Clover shaped Standard length Has an amino acid binding site anticodon tRNA has H bonds between complementary base pairs Limited number of types (64) mRNA Linear Variable length (depends on the length of gene) Many different types (depends on the gene) No H-bonding No base pairs
Compare DNA Vs RNA
Similarities: Contain phosphate Made up of nucleotides Contains organic bases (A, C and G) (not T as it is replaced by U in RNA) Pentose sugar Differences RNA single stranded RNA has non-coding strands (introns) removed Ribose sugar in RNA deoxyribose in DNA U in riobose replaces the T 3 types of RNA, only one DNA Smaller than DNA
Compare the structures of RNA and DNA;
Alike both have phosphate/phosphoric acid/PO4; bases/named bases/accept letters; nucleotides; pentose sugar; Different DNA deoxyribose; DNA thymine; DNA double stranded; DNA larger/longer; DNA one form RNA 3 types;
Describe translation and transcription
Transcription Section of DNA unwinds / uncoils; DNA separates, h-bonds break RNA nucleotides align; complementary base pairing / example of pairing; U replaces T mRNA polymerase (joins nucleotides); mRNA is modified, introns are removed Translation mRNA moves into cytoplasm / through nuclear pore / to ribosome; tRNA carries specific amino acid; mRNA read in codons / triplets; anticodon of tRNA matches codon of mRNA; ATP used in activation / joining amino acids; amino acids join by peptide bonds; tRNA used repeatedly; sequence of bases / codons determines sequence of amino acids;
Describe what happens during translation.
Codons on mRNA; anticodons on tRNA; 20 types tRNA molecule specific amino acid attached to tRNA; peptide bonds formed
Describe the role of tRNA in the process of translation.
Anticodon complementary to codon/reads message on mRNA;
Specific amino acid;
Carried/transferred (to ribosome);
Correct sequence of amino acids along polypeptide;
Comparison of replication and transcription
Similarities
H bonds break and the DNA unzips
DNA acts as a template for complimentary base
Polymerase enzymes are involved
Differences
U replaces T in RNA
In replication all the DNA is copied, in transcription on sections are copied
Only one strand is used as a template in transcription (antisense strand), both strands are used in replication
RNA polymerase in transcription whereas DNA polymerase is sued in replication
mRNA is produced in transcription, DNA is produced in replication