DNA Flashcards

1
Q

DNA stands for

A

deoxyribonucleic acid

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2
Q

Nucleotides are made up of

A
  • a sugar called deoxyribose
    -a phosphate group called Po-4
    -and organic nitrogen base
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3
Q

DNA is

A

present in all living organisms

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4
Q

DNA controls

A

all the chemical changes within a cell.

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5
Q

The organic bases of DNA bases are

A

adenine
cytosine
thymine
guanine

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6
Q

ribose

A

Ribose is a sugar, like glucose, but with only five
carbon atoms in its molecule.

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7
Q

DNA gyrase

A

Is an enzyme relaxes the molecule ( uncoil)

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8
Q

DNA helicase

A

breaks the hydrogen bonds separates the two strands

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9
Q

RNA primase

A

single stranded it searches for a base code and adds an RNA primer.

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10
Q

DNA Polymerase lll

A

searches for and attaches at the RNA primer site once attached the DNA polymerase III begins adding free matching nucleotides to the original 3’ strand.

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11
Q

Complementary base pairing means that two bases are always each other’s pair.

A

true

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12
Q

The only way Watson and Crick’s model would work is if purines paired with other purines and pyrimidines were paired with other pyrimidines.

A

false

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13
Q

There are 8 organic base pairings for every complete 360 degree twist of the DNA molecule.

A

no there are 10

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14
Q

Rosalind Franklin and Maurice Wilkins used x-ray diffraction, where strands of DNA were bombarded with X-rays to yield a pattern on photographic film.

A

true

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15
Q

The nitrogenous base is attached to the 1’ carbon of the sugar by a glycosyl bond and the phosphate group is attached to the 5’ carbon by a phosphodiester bond.

A

true

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16
Q

the nitrogens are numbered

A

clockwise

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17
Q

In the carbon based deoxyribose sugar, the first four of the five carbon atoms, together with an oxygen atom, form the 5-membered ring. The fifth carbon sticks out from the ring.

A

true

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18
Q
A
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19
Q

replication can occur…

A

at different sites within one DNA molecule at the same time

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20
Q

enzymes are

A

proteins that perform a function

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21
Q

DNA HELICASE…

A

breaks the hydrogen bonds holding the strands together

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22
Q

what is the difference between dna and rna

A

dna is double stranded forming a double helix while rna is usually single stranded

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23
Q

DNA ligase

A

Seals the gap

24
Q

DNA polymerase 1

A

replaces the rna primer with proper DNA. nucleotides

25
DNA Polymerase 2
proof reads the finished molecule; corrects mistakes
26
adding nucleotides goes
5' to 3'
27
RNA is a
single chain
28
DNA has _______ sugar
deoxyribose
29
RNA has _______- sugar
ribose
30
RNA can be found in
nucelus and cytoplasm
31
DNA can be found in
the nucleus
32
are there different types of DNA
no
33
are there different types of RNA
MRNA TRNA rRNA
34
okazaki fragments are found on the
lagging strand
35
transcription occurs in
nucleus
36
translation occurs in a
ribosone
37
crispr allows scientists to
figure exactly where dna was cut
38
crispr Jean editing what is it
it allows scientists to make changes to DNA
39
how does crispr work
based on a bacterial immune system that allows a bacteria to fight an infection it uses an enzyme called CAS9
40
Franklin
disovered the structure of dna ( double helix)the famous photo is called " photo 51"
41
chargaff
discovered the base pairing in DNA . he discovered the 4 niteogenous bases and declared the chargaff rules.
42
substitution
a mutation that exchanges one base for another
43
insertion
are mutations in which extra base pairs are inserted into a new place in the DNA
44
deletion
mutations in which a section of DNA is lost or deleted
45
Wilkins
he began the x ray diffracrion images of DNA that contributed to Watson and cricks discovery
46
frame shifts happen due to
deletion or insertion multiple bases are affected
47
point mutations
when only one nucleotide is altered the mutation if referred to as a point mutation
48
translocation mutations
if a longer sequence of nucleotides are changed, the mutation is referred to as a translocation mutations.
49
missense mutations
change a codon to one that encodes a different amino acid and cause a small change in the protein produced
50
silent mutations
change a codon to one that encodes the same amino acid and causes no change in the protein produced.
51
nonsense mutations
change a amino acid coding codon to a single " stop" codons. and cause an incomplete protein. This can have serious affects since the incomplete protein probably wont function
52
causes of mutations
DNA fails to copy accurately
53
what is hemoglobin
Hemoglobin is a protein containing iron that facilitates the transport of oxygen in red blood cells
54
ssbs do what
hold strands of DNA apart
55
DNA Polyermase III
searches for and attaches at the primer site and then begins adding free matching nucelotides
56
RNA polymerase