DLA 23: Connective Tissue

Question 1

Transcient cell population

Answer 1

1) Lymphocytes
2) Plasma cells
3) Basophils
4) Neutrophils

Question 2

Permanent residents of connective tissue

Answer 2

1) Fibroblasts
2) Adipose cells
3) Macrophages/monocytes
4) Mast Cells
5) Mesenchymal stem cells

Question 3

Fibroblasts

Answer 3

-Synthesize fibers and ground substance

• Fibroblasts = active
• Fibtocytes = inactive
• myofibroblasts = wound healing

Question 4

Adipocytes:

Unliocular vs Multiocular

Answer 4

Uniocular Adipocyte

• One large “white” fat droplet
• Large fat inclusion (organelles nucleus pushed to PERIPHERAL of the cell)
• Major energy source (triglycerides)
• Rich blood supply

Multiocular Adiocytes

• Many “brown” lipid droplets
• CENTRAL nucleus
• Rich in mitochondria
• Lipochrome pigment
• Main functions:
  1) Heat production
• Specialized locations in the body neck, abdomen of neonates

Question 5

Macrophages

Answer 5

-Derived from monocytes, migrate to connective tissue

• Macrophages in LIVER = kupffer cells,
• Macrophages in BRAIN = microglia
• Macrophages in BONE = oseoclasts

Question 6

Mast Cells

Answer 6

-Originate in bone marrow

-Gain cytoplasmic granules when reach connective tissue
(Granules will stain another color different from metachromatic dye, also PAS positive bc glycoproteins nature)

-Mast cells and basophils both derived in bone marrow and circulate the blood

Question 7

Lymphocytes

Answer 7

• Small/spherical
• Condensed basophilic nucleus
• Immune cells (B and T)
• B-lymphocytes develop into plasma cells

Question 8

Plasma cells

Answer 8

• Derived from B-lymphocytes

- Synthesize and secretes glycoproteins (a immunoglobulin)

Question 9

Eosinophils

Answer 9

• Red granules in the cytoplasm
• Condensed bilobed nucleus
• Phagocytosis of antibody
• Kills parasitic worms

Question 10

Elastic Fibres

Answer 10

3 Stages of development:

1) Oxytalan
2) Elaunin
3) Elastic

Types:

1) Fibrillin (glycoproteins) + elastin (protein)
2) Elastin (Glycine, proline, lysine, desmosine, isodesmosine)

• Very elastic
• Found in large arteries, elastic cartilage, vocal ligament, bronchi, etc

-Requires Orecein, Resorcin, or Verhoeff’s staining

Question 11

Interstitual fluid

Answer 11

1) Small quantity of H2O for solvation and diffusion

2) Edema- Body parts swell from injury or inflammation