Disorders of Calcium and Phosphate Metabolism Flashcards
1.Explain how osteoblast differentiation is induced by the RANK ligand?
• RANK (receptor activator of nuclear factor kappa-B) surface receptor on pre-osteoclasts stimulates osteoclast differentiation when RANK-L binds to it.
o RANK-ligand: produced by pre-osteoblasts, osteoblasts and osteocytes.
• OPG (osteoprotogerin): decoy receptor produced by osteocytes that binds to RANK-L to prevent the activation of RANK and so inhibits osteoclast differentiation.
- What inhibits osteoclast differentiation?
OPG (osteoprotogerin): decoy receptor produced by osteocytes that binds to RANK-L to prevent the activation of RANK and so inhibits osteoclast differentiation.
- What is the WNT signalling pathway?
- A highly conserved, complex signal pathway that is involved in animal development - Drosophila wingless gene (1987)
- WNT binds to the frizzled receptor which requires the co-factor LRP5/6 to become activated. The activated receptor activates B-catenin which migrates to the nucleus to bind to transcription factors (e.g. TGF)- osteoblast differentiation
- What is the WNT negatively signalled by?
DKK (dickkopf) and sclerostin (SOST).
- What is osteoCLAST differentiation:
- Promoted by
- Inhibited by
- Promoted by RANK-L/M-CSF
- Inhibited by NO/OPG.
- What is osteoBLAST differentiation :
- Promoted by
- Inhibited by
- Promoted by NO/ATP/PGE2
- Inhibited by SOST/DKK1/SFRP1
- Calcium homeostasis has already been covered in another lecture so a quick overview
- What % of body calcium is in bone and intracellular
- What amount of extracellular plasma is there?
- How much is bound, how much is free?
- What maintains Ca balance
- 99% of body calcium is in bone and the remaining 1% is mainly intracellular
- Extracellular: plasma Ca 2.2-2.6 mmol L-1
- About half is free [Ca2+] (physiologically active), half protein bound (mainly albumin).
- Hormonal control of the tiny (<0.1%) extracellular fraction is what maintains Ca balance.
- Where is Ca absorbed from and how?
•Ca is absorbed from the gut using vitamin D which is produced by PTH (PTH causes Ca reabsorption from the kidneys/gut and release from the bone).
- What % of the bodies phosphorus is in the bone?
•85% of body phosphorus is in bone and remainder is mainly intracellular
- What form is extracellular phosphorus in?
H2PO4^-, HPO4^2-
- What amount of plasma Phosphate should there be? What is an appropriate reference range?
2.5 - 4.5 mg dL-1 (0.75-1.45 mmol L-1)
- Where is Pi absorbed from and how?
•Pi is absorbed from the gut via vitamin D which is produced by PTH (PTH causes Pi excretion from the kidneys).
- How is Pi regulated by FGF-23?
•Pi is also regulated by FGF-23 from osteoclast which promote Pi excretion and inhibits vitamin D activation.
- Case Study:
A 75 year old widow was investigated for bone pain and muscle weakness. She told the GP that she was vegetarian and rarely left her home.
Serum investigations: Total calcium 1.82 mmol/l (2.20 - 2.52) Phosphate 0.70 mmol/L (0.75 - 1.50) Albumin 39 g/L (35 - 48) Alkaline phosphatase 187 U/L (30 - 100) Creatinine 69 umol/L (60 - 110)
Interpret these results?
LOW calcium
LOW phosphate
HIGH alkaline Phosphatase
• Vitamin D deficiency (diet and sunlight) causes low Ca and Pi – increase PTH to try to increase vitamin D.
- What is another name for vitamin D?
Calcitriol