Disinfectants

Question 1

Which disinfectant(s) are affected by organic matter

Answer 1

Alcohols, biguanides, chlorine compounds

Question 2

Which disinfectant(s) degrade RNA, DNA

Answer 2

Alcohols, chloride compounds, peroxygens, silver, QAC

Question 3

Name the mechanism of peroxygens as disinfectants

Answer 3

ROS attack lipids, proteins, enzymes, DNA by disrupting SH and S-S bonds

Question 4

Which disinfectant(s) involves the leak of intracellular proteins or a.a.

Answer 4

Chlorine compounds, diamidines, phenols, QAC

Question 5

Name the mechanism of biguanides as disinfectants

Answer 5

Crosses bacterial outer membrane to attack cytoplasmic or inner membrane

Question 6

Name the mechanism of phenols as disinfectants

Answer 6

Leakage of intracellular contents including K+

Question 7

Which disinfectant(s) work by inhibition of protein synthesis

Answer 7

Alcohol, aldehyde, chlorine compounds, iodine compounds, mercuric chloride

Question 8

Which disinfectant(s) work by inhibition of DNA, RNA synthesis

Answer 8

Aldehydes, chlorine compounds, iodine compounds

Question 9

Which disinfectant(s) are sporicidal

Answer 9

Aldehydes, chlorine compounds, peroxygens

Question 10

Name the mechanism of mercuric chloride as disinfectants

Answer 10

Affect microbial peptide synthesis

Question 11

Which disinfectant(s) interact with the SH/S-S groups in proteins, enzymes and DNA

Answer 11

Aldehydes, chlorine compounds, peroxygens, silver

Question 12

Name the mechanism of alcohols as disinfectants

Answer 12

Inhibits the production of metabolites required for rapid cell division. Denatures proteins

Question 13

Name the mechanism of aldehydes as disinfectants

Answer 13

Interact with SH, OH, carboxyl and amino groups to alter protein, RNA, DNA synthesis

Question 14

Name the mechanism of iodine compounds as disinfectants

Answer 14

Disrupt protein and nucleic acid structure and synthesis

Question 15

Name the mechanism of silver as disinfectants

Answer 15

Interacts with SH group in enzymes and proteins.

Affect microbial plasma membrane, cytoplasmic membrane and nucleic acids.

Question 16

Name the mechanism of QAC as disinfectants

Answer 16

Reaction with cytoplasmic membrane followed by membrane disorganisation.

Leakage of intracellular contents. Degradation of proteins, RNA, DNA. Wall lysis by autolytic enzymes

Question 17

Name the mechanism of diamidines as disinfectants

Answer 17

Inhibit oxygen uptake.
Leakage of a.a.

Question 18

Name the mechanism of ammonia as disinfectants

Answer 18

Soaponifies lipid components of microorganism envelope

Question 19

Which disinfectant(s) degrade proteins

Answer 19

Alcohols, peroxygens, mercuric chloride, silver compounds, QAC

Question 20

Name the mechanism of chlorine compounds as disinfectants

Answer 20

Oxidation of SH groups & enzymes in a.a, ring chlorination of a.a.

Leakage of intracellular contents, DNA breakages.

Inhibition of oxygen uptake, protein synthesis, decreased nutrient uptake, ATP production, DNA synthesis.