Direct and Quenched

Question 1

Characteristics of Direct methods? (5 characteristics)
What is rare and limited in direct methods? (2 things)

Answer 1

Reaction observed as it happens
Non-invasive (nothing added to arrest reaction)
Reaction is probed using techniques that are specific to chemical of interest
Simpler and quicker than quenching
Fewer steps means less chance of error

Rare to be able to monitor all reaction components simultaneously
Types of reactions that can be measured directly is limited

Question 2

3 steps of Quenching?
- How many sets of instruments per step?

Answer 2

1. Starting (triggering) and stopping (quenching) the reaction
2. Separation of substrates and products
3. Quantification and Identification

• One set of instruments per step

Question 3

Characteristics of Quenching methods? (3 characteristics)
Drawbacks? (1 drawback)

Answer 3

Reaction is arrested and the extent of the reaction is assessed at each timepoint
Reaction arrested by quenching agent
Allows for measurement of multiple components; Less limited than direct

More error prone to due to more steps