Differentiation, Development and Apoptosis Flashcards
1
Q
Provide a brief overview of cellular development
A
- Genes are not gained of lost in the normal course of development
- Their expression is controlled
- Differentiation is determined by the selective expression of genes within a cell
- Thus different cells express different proteins
- Cells commit to certain fates
2
Q
What is differentiation and how does it occur in a spatio-temporal manner
A
- Cells differentiate to carry out specialised functions, produce specific proteins
- Preceded by rapid proliferation
- DNA orchestrated set of cellular changes that normally occurs without error
- Development of specialised cells recognised morphologically
- Regulation of gene expression in a spatio-temporal manner (integration / coordination of events)
- Spatial: Cell fate / differentiation patterned in space
- Temporal: Involves the intricate patterning & timing of cell proliferation, activation of cell division in some regions, imposition of cell cycle arrest in others
3
Q
What is symmetric vs asymmetric division
A
- Symmetric: Yields identical daughter cells that may have different fates if exposed to different external signals
- Asymmetric: Yields two different types of daughter cells with different fates, asymmetrical localisation of cell fate determinants lead to unequal daughters cells
4
Q
What are stem cells and stem cell niches
A
- Undifferentiated cells that may or may not be committed to a particular fate
- Give rise to more stem cells (self renewal) and generate differentiated progeny
- Present at all stages of development
- Maintain stem cell population, increase stem cells or increase differentiating cells
- Stem cells are formed in niches that provide signals to maintain a population of undifferentiated stem cells but prevent excess proliferation
- Stem cells regenerate differentiated tissue cells that are damaged, sloughed, or aged
- Most stem cells are multi-potent and can undergo symmetric or asymmetric self-renewal divisions
5
Q
What are the types of stem cells
A
- Totipotent:Ability to give rise to a new individual, given adequate maternal support(fertilised egg)
- Pluripotent: Ability to give rise to a wide range of somatic cells / tissues
- Multipotent:Can develop into a few cell types (blood, muscle, nerve, bone)
6
Q
What are embryonic stem cells
A
- Embryonic blastocyst inner mass cells are pluripotent
- Give rise to all differentiated cell types of the organism
- Pluripotency is controlled by
state of DNA methylation, chromatin regulators, certain micro-RNAs, and TF (Oct4, Sox2, and Nanog) - Can be isolated, cultured and maintained / grown indefinitely to form differentiated cell types
7
Q
What are somatic stem cells
A
- Considered to be multipotent, limited capacity to divide (lack telomerase activity)
- Maintain / regulate homeostasis and repair / regeneration
8
Q
What are induced pluripotent stem cells (iPS)
A
- Formed from somatic cells by expression of key TFs
- Treatment with therapeutic compounds, transplant genetically matched healthy cells
- Wound healing, blindness, deafness, stroke, bone marrow, spinal cord injury, arthritis, diabetes, cancer
9
Q
What is the difference between differentially expressed and constitutively expressed genes
A
- DE: Inducible genes, only turned ‘on’ when needed, regulated
- CE: Housekeeping genes, always turned ‘on’, continually transcribed
10
Q
What mediates development
A
- Transcriptional Regulation: inducible / housekeeping genes
- Cell Signalling: Direct cell-cell contact, soluble factors released by cells (morphogens)
11
Q
How do gradients of maternally derived regulatory proteins establish polarity of the body axis and control transcriptional activation of zygotic genes
A
- Mother deposits material (mRNA and protein) that creates asymmetries and set up gradients that broadly define areas (basic body plan map)
- Gene interaction subdivides these areas (cells differentiate)
- These identities are remembered, asymmetries at poles, chemical longitude and latitude system
- Subdivide territory into broad domains and create finer subdivisions and commit them to memory
- Establish anterior-posterior (segments), dorsal-ventral (germ layers)
12
Q
What is apoptosis
A
- Programmed cell death that is a normal and necessary event of normal development
- Triggered by variety of signals, active, physiological / pathological
- No inflammation, cell shrinkage
- Fragmentation / condensation of chromosomes / cytoplasm
- Organelle disruption
- Fragmentation of cell, sequential destruction of cell
- Release membrane bound fragments, phagocytosis
13
Q
What is necrosis
A
- Death due to unexpected and accidental cell damage (toxins, radiation, heat, trauma, hypoxia)
- Swelling, holes appear in the plasma membrane, intracellular materials spill into surrounding environment
- Causes tissue damage, inflammation, oedema, recruitment of WBC’s
- Passive, pathological, inflammation, cell / mitochondrial swelling
- DNA degradation, breakdown of plasma membrane, loss of ion transport, cell lysis / dissipation
14
Q
How is cell death regulated
A
- Cells have intrinsic apoptosis pathways for suicide without release of cytosolic contents
- Crucial for normal development
- Require trophic factors that bind surface receptors to repress apoptosis
- Balance between life and death
- Involves activation of cellular caspase proteases (death signals)
15
Q
What is the function of apoptosis
A
- Embryogenesis, morphogenesis, cell selection, immunity
- Tissue remodelling, maintaining organ size / shape
- Protection against cancer, p53 / inducing apoptosis
- Cells lost are replaced by mitosis
- Occurs in all multicellular organisms
