Diagnostics Flashcards
1
Q
Why do we identify infectious disease?
A
- Knowledge of likely spread
- Epidemiology- protect other animals
- Choice of treatment- resistance to antibiotics, change the treatment
2
Q
How can we diagnose infection?
A
-Grow the agent
-Look for a response to the target
-look for DNA/RNA of the agent
-Look for pathological signs
Each method has its own strengths and weaknesses.
3
Q
Considerations when growing an infectious organism
A
- Is it aerobic or anaerobic
- can it cause disease or is it a contaminant
- How quickly does it grow?
- Can I grow it at all?
4
Q
Pros of growing an infectious agent
A
- Know you have a live organism
- positive identification
- No prediction is necessary
- Can test it for antibiotic resistance
5
Q
Cons of growing infectious agents
A
- False positives are common for some diseases
- Slow, by the time it;’s grown the animal could be dead.
6
Q
Looking for a response
A
- Dipstick test= lateral flow device
- ELISA- antigens and antibodies
- Microscopic agglutination test, take a serum from an animal with a known bacteria and see if it goes clumpy
7
Q
Looking for a response 2
A
- Can look for an antigen circulating in the blood, using an antibody to capture this
- can look for antibody- IgG or IgM
- Binding makes complexes: MAT visible, detected by a second antibody once it’s detected
8
Q
who is ELISA
A
