diagnostic and typing methods Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

name 3 bacteria associated with periodontal disease

A

p.gingivalis
prevotella intermedia
tanerella forsythia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is the main bacteria implicated in dental caries

A

streptococcus mutans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

name a bacteria that may be implicated in root canal infections

A

porphyromonas endontalis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

microbiological culture methods of bacterial detection

A

traditional method
bacteria are grown on a suitable agar medium then isolated and identified

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what 3 tests can be done once bacteria have been isolated from agar to identify them

A
  • anaerobes can be detected using metronidazole disc ( this kills anaerobes)
  • gram stain (gram positive stain dark purple)
  • Rapid API 32A - kit allowing identification from enzyme activity and sugar fermentation profile of bacteria
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

disadvantages of using microbiological culture methods for bacteria detection

A

labour intensive, requires viable cells, relatively insensitive, only small numbers of samples can be analysed at once, often inconclusive results

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

name two types of molecular biological methods of bacteria detection

A

DNA probes
PCR
PCR - RFLP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

DNA probes

A

single stranded sequence of DNA that searches for its complementary sequence within a sample
probes are labelles which an agent e.g fluorescent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is the probe type of choice if doing DNA probing

A

oligonucleoride probes. - small size making them very specific

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what gene do all bacteria process

A

16S ribosomal gene
contains 9 variable regions that contain unique DNA sequences for each individual bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

polymerase chain reaction

A

exponential amplification procedure whereby a single molecule of DNA can be amplified billions of times to produce a large amount

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

name 3 advantaged to using molecular biological methods of bacterial detection compared with microbiological culture methods

A

less time consuming
very sensitive
dont require viable cells
dont need to be analysed immediately
can directly detect bacteria from within clinical samples

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

molecular typing

A

identifying different strains of micro organisms by looking at their genetic material

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what is the ultimate typing method

A

DNA sequencing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

name 4 methods of collecting a specimen from a patient

A

smear (scrape)
oral rinse
swab
biopsy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly