development of neurotransmitters

Question 1

what would be observed if diptheria toxin was knocked into GDF7 loci?

Answer 1

initial differentiation unaffected
then roof plate removed
WT: Ngn1 and Math1 expressed at dorsal neural tube. Mash1 is expressed but not at the dorsal region.
roof plate removed: spread of mash1 expression dorsally. no ngn1 and math1 expression.

Question 2

dlLA and dlLB neurons

Answer 2

innervated by primary sensory neurons from DRG

innervate spinal trigeminal nuclei in the hindbrain

Question 3

which gene must a class B progenitor express for it to become an inhibitory dILA neuron?

Answer 3

ptf1a

Question 4

which gene must a class B progenitor express for it to become an excitatory dILB neuron?

Answer 4

gsx1/2

Question 5

what transcription factors do dlLB neurons express?

Answer 5

Lmx1b

Tlx3

Question 6

in dILA neurons where only Pax2 is maintained, what neurotransmitters are expressed and how does Pax2 lead to their expression

Answer 6

Pax2 turns on NeuroD1/2/6

NeuroD1/2/6 turns on expression of galanin and dystrophin

Question 7

in dILA neurons where both Pax2 and Lhx1/5 is maintained, what neurotransmitters are expressed and how does Pax2 and Lhx1/5

Answer 7

Pax2 and Lhx1/5 co-operatively turn on expression of encephalin and NPY

Question 8

what transcription factors do dlLA neurons express?

Answer 8

Pax2

Lhx1/5