detecting proteins using antibodies

Question 1

what are immunoassay techniques used for?

Answer 1

Immunoassay techniques are used to detect and identify specific proteins

Question 2

what do immunoassay techniques use?

Answer 2

These techniques use stocks of antibodies with the same specificity, known as monoclonal antibodies

Question 3

what is linked to a chemical label?

Answer 3

An antibody specific to the protein antigen is linked to a chemical ‘label’

Question 4

what is western blotting?

Answer 4

Western blotting is a technique, used after SDS–PAGE electrophoresis

Question 5

describe western blotting?

Answer 5

The separated proteins from the gel are transferred (blotted) onto a solid medium. The proteins can be identified using specific antibodies that have reporter enzymes
attached.