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M2M1 > Details > Flashcards

Details Flashcards

1
Q

alpha-amanitin

A

non competetive inhibitor of RNA pol II - bind to bridge helix and blocks translocation

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2
Q

Rifampicin

A

blocks RNA exit channel of bacterial RNA polymerase

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3
Q

TFIIH clinical syndromes

A

XP
CS
TTD

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4
Q

TFIID

A

Binds TATA box

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5
Q

TFIIH

A

helicase activity and CDK7

Functions in both transcription and DNA repair

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6
Q

Abnormal CD44 splicing –>

A

Cancer

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7
Q

Example of antisense oligo rescue

A

spinal muscular atrophy

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8
Q

Promoter diseases

A

Beta thalassemia
Hemophilia B leyden
Fragile x (trinucleotide repeat)

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9
Q

Craniosynostosis

A

Homeodomain MSX2 - DNA binding domain - binds tighter

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10
Q

Androgen insensivity

A

Zinc finger AR - either AR BD or DNA BD - less

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11
Q

Waardenburg Syndrome

A

bHLH - MITF - melanocyte development and hearing - less

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12
Q

SWI/SNF (use ATP)

A

Disrupt octamers

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13
Q

N- termini histones

A

lysine

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14
Q

Somatic mutations in SWI/SNF genes –>

A

Cancer

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15
Q

Rubinstein Tabi

A

Haploinsufficieny of CBP (a HAT / transcriptional coactivator)

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16
Q

Leukemia and Gene Expression

A

Abnormal fusion proteins - HATS or HDACS with transcriptional regulators –> improper expression

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17
Q

SSBP regulation (5)

A 
ligand
nuclear entry
dna access
phosphorylation
degradation/transcription
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18
Q

How does cyclosporine work?

A

It inhibits calcineurin
Thus, calcineurin can’t de-P NFAT
NFAT nls remain hidden
NFAT can’t go to nucleus to transcribe immune response

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19
Q

Example of regulating TF by amount

A

B-catenin
normally ubiquitinated sent to proteosome
wnt interrupts GSK3 APC/Axin - don’t P B-catenin
build-up
go transcribe

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20
Q

If we mutate APC what happens

A

Colon cancer

too much beta catenin transcribing (with TCF) too many wnt genes

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21
Q

p53 regulated via?

A

the amount

MDM2 targets for destruction and masks activation domain

22
Q

Example of regulation via DNA binding

A

ID proteins and HLH - prevent DNA binding b/c lack basic domain

23
Q

Example of regulation via P

A

CREB - normally bound to DNA but inactive - P results in activation and recruitment of CBP (HAT) –> RNA Pol II

24
Q

4E-BP

A

Sequester EIF4E and block fx

25
Q

mTOR

A

Phosphorylates 4E-BP -
preventing 4E binding EIF4E
Enabling translation

26
Q

Rapamycin

A

Dephosphrylates 4EBP
Enabling 4EBP binding EIF4E
Disabling translation

27
Q

Interferon

A

phosphorylates EIF2-alpha

preventing initiation of translation

28
Q

EIF2-alpha necessary for

A

inition of translation via tRNA met ribosome binding

29
Q

Transferrin

A

Binds iron

30
Q

Transferrin Receptor

A

Binds Transferrin/Fe and brings into cell

31
Q

Transferritin

A

Intracellular Fe holding tank

32
Q

Iron response element

A

Loop in mRNAs that can bind Iron response element Binding proteins

33
Q

Iron response element binding proteins

A

bind iron and then bind IRE regulating translation

34
Q

When iron is high what happens with transferritin?

A

When Iron is high, the IRE-BP cannot bind to the IRE of the mRNA for transferritin and we get increase translation - we need more storage

35
Q

When iron is high what happens with transferrin receptor?

A

When iron is high, the IRE-BP doe not bind to the IRE of mRNA for transferrin receptor and will decrease translation/ degrade mRNA - we need less

36
Q

Gleevac

A

Bcr-abl tyrosine kinase inhibitor (CML)

37
Q

Bortezomib

A

Proteosome inhibitor (Multiple myeloma)

38
Q

Crixivan

A

HIV protease inhibitor

39
Q

Captopril

A

ACE inibitor

40
Q

helix breakers

A

proline and glycine

41
Q

err in posttranslational hydroxylation of proline?

A

Scurvy (collagen)

42
Q

err in Gly–>Cys mutation in collagen?

A

Osteogenesis imperfecta

43
Q

HSP40/70

A

Prevent hydrophobic aggregation

44
Q

Chaperonin

A

GroES + 2x GroEL –> barrel that helps fold proteins

45
Q

protein disulfide isomerase

A

rearrange to correct s-s bonds

46
Q

peptide prolyl isomerase

A

increase rate of conversion or proline to cis

47
Q

Cyclosporin

A

Prevents calcinuerin from dephosphorylating NFAT - can’t move into nucleus to transcribe immune response

48
Q

Puromycin

A

tRNA mimic

translation terminator

49
Q

Cisplatin

A

base alkylator

50
Q

acitomycin D and Doxorubicin

A

Intercalators

51
Q

Etoposide and Captothecin

A

Topoisomerase targeters

M2M1 (7 decks)

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