Dental Caries Flashcards
Dental caries - Definition?
- a bacterial disease of the calcified tissues of the teeth characterised by the demineralisation of the inorganic and destruction of the organic substance of the teeth
Dental caries - Acidogenic theory of dental caries? What happens? What’s affected?
What happens:
- result from a metabolic shift accompanied by gradual change in ecology of the dental biofilm
- imbalance in equilibrium between tooth mineral and biofilm fluid develops
- acid produced by oral bacteria from carb fermentation
What’s affected:
- decalcification of hard tissue substance and disintegration of the organic matrix
Dental caries - Aetiology of caries? Primary and secondary modifying factors?
Primary: - morphology - saliva - pH - fluoride - diet - hygiene - immune - genetic Secondary: - socioeconomic - education - lifestyle - environment - age - ethnic group - occupation
Dental caries - Bacterial metabolism? Acquired pellicle? Dental plaque? Defintion?
Acquired pellicle:
- absorbed layer of mainly salivary glycoprots that forms on clean enamel, which bacteria adheres to
Dental plaque:
- biofilm of bacteria embedded in matrix of salivary mucins and extracellular polysacchardie polymers (on all surfaces)
Dental caries - Development of dental biofilms - process?
Process:
- pellicle forms
- attachment of bacterial colonisers (0-14h)
- co-adhesion and growth of attached bacteria leading to microcolonies (4-24h)
- microbial succession leading to increased bacterial diversity with continued adhesion and growth (1-7d)
- climax community/mature biofilm (1 week)
Dental caries - Initial colonisers - types? content? nutrients?
Types: - S.sanguis/oralis and mitis - Actinomyces and Neiserria Content: - 95% strep - 56% total microflora Nurients: - endog molecules of saliva
Dental caries - Microbial succession - early colonisers to bridge?
- from strep dominated to a plaque dominated by actinomyces
- bacteria produce polysaccaride and these contribute to biofilm matrix
Dental caries - Mature biofilm - diverse composition? Organisms reducing acidogenic potential?
Diverse composition: - facultative or obligate anaerboes - +ve bacteria (low pH non-mutans strep, mutants strep, actino and lactobacillus) Reducing potential (organisms) - veillonella app - S.sangius - S.salivarius - A.naeslundii
Dental caries - Cariogenic features of dental biofilms bacteria - characterisitics?
- rapidly transport fermentable sugars
- conversion of sugar to acids
- maintain sugar metabolism under extreme conditions (low pH)
- prod of extra/intracellular polysacc
Dental caries - S. mutans - absence and high level effects?important role?
Absence: - caries can develop High levels: - caries may not develop Important role: - role in caries initiation (lactobacillus for progression)
Dental caries - Lactobacillus - type? characteristics? location?
- pioneer organism
- +ve non-spore forming rod (microaerophillic)
- colonise dorsum of tongue
- progression of caries
- established loss pH increases number of lactobacillus and reduces mutans
- contributes demineralisation of teeth once lesions established
Dental caries - Oral actinomyces - characteristics?
Characterisitics:
- +ve non spore, rods and filament
- good plaque former
- ferment glucose and produce lactic acid
- A. viccosus and A. naeslundii (root caries)
Dental caries - Summary of the microbiology of dental caries - overview? Ecological plaque hypothesis?
- S.mutans resident flora, but very low numbers (high in white spot lesions)
- S.mutans and Lactobacilli does not explain variation in caries experience
- Many acid-prod microorganisms and so no single organism can be attributed to caries
Ecological plaque hypoth: - consequence of changes in balance of resident microflora
Dental caries - Intrinsic factors that influence tooth caries?
- Enamel composition
- Enamel structure
- Tooth morphology
- Tooth position
Dental caries - Extrinsic factors that influence tooth caries?
- Saliva
- Diet
- Fluoride
- Immunity
Dental plaque - resting pH? pH changes with sugar? rate changes?
Resting pH:
- 6.5 - 7
pH changes:
- falls to around pH 5 within 2-3 minutes of rinsing the mouth with sugar solution (20 mins to go back to normal)
Rate changes:
- speed in pH drop indicated of speed of plaque metabolism
Dental plaque - how sugar reduces pH? Critical pH?
How sugar reduces pH:
- dietary sugars diffuse through plaque
- converted to lactic acid, acetic and propionic acids by bacteria
- pH could fall by 2 units in 10 mins
Critical pH:
- 5.5 is the lowest the pH can drop before demineralisation can occur
Plaque - action of bacteria?
Action of bacteria:
- store glucose as glycogen (intracellular store)
- S.mutans synthesise other polymers given access to sucrose
- can synthesise extracellular polymers from simpler sugars
- increase plaque bulk and are more cariogenic
- glucosyltransferase break down sucrose into fructose and glucose, using this energy released to build glucose and fructose polymers
- glucans and fructans deposited extracell providing sticky environment for plaque organism
- ability of microorganisms to form plaque related to ability to synth polysacc
- control targeted at GST
Dental caries - The effect of diet on caries - factors? sugars? sucrose?
Factors:
- plaque composition influenced by diet
- S.mutans greater with high sucrose diets
- lactobacilli is acid tolerant and found in greater proportions in carious cavities and deep layers of plaque exposed freq to sugars
Sugars:
- sucrose, glucose, fructose, maltose, lactose and galactose
Sucrose:
- affects the composition of early plaque, leading to higher numbers of bacterial species capable of synth glucan from sucrose
- plaque bulkier with sucrose-rich diet in comparison
- encourages dextran prod in plaque due to energy released (sucrose broken down into 2 monosacc)
Dental caries - Substitutes to dietary sugars - effects? Xylitol?
- Non-sugar sweeteners are virtually non-cariogenic
- Xylitol though to prevent caries
- Sugarless gum prevent caries by stimulating salivary flow
Xylitol: - affects bacteria growth and metabolism
- affects de/mineralisation
- reduces biofilm form
- decreases S.mutans
Dental caries - Oral pH change - on eating food item?
- Food item containing sugars, rapidly fermented by acidogenic bacteria, rapid acid production, plaque pH will fall
- other items eaten, before, during or after influence pH
- stimulates saliva (raising pH)
- remineralisation may be enhanced by Ca or fluoride (from food)
Dental caries - Key factors for primary dentition caries?
- Sticky products are more cariogenic
- Sweetened pacifiers
- Sweetened drinks in bottles
- Prolonged breast feeding
Fluoride impact on plaque and dental caries?
- High fluoride conc in plaque
- Fluoride favours precip of Ca and Pi
- Ca/Fl apatite encouraged
- Fl-apatite form part of development (if administered)
- Less soluble in acids more stable crystals
- Fl ions inhibit bacterial metabolism
Dental caries - Substitutions in the hydroxyapatite crystals? Effect?
Main substituents of apatite: - HPO4, CO3 for PO4 - Sr, Ba, Pb, Na, K, Mg for Ca - F, Cl, Br and I for OH Ions present may influence ability of formation of dental caries - Fl inhibits cires - CO3 promotes carious attack