deck1 Flashcards
what is the name of the oldest plant organism in the world? how old is it? where is it located
bristlecone pine, 4,700 years old, rocky mountains
discuss the modular construction of plants
the plant is made up of repeating units called modules (node, internode, lateral bud, leaf)
discuss the two different types of organs plants have
vegetative organs (used for growth);
roots with root hairs which are used for water uptake
shoots used for growth and contains xylem/phloem
reproductive organs
discuss root structure
lateral roots from primary root are used for anchorage
what are the three tissue types in a plant
dermal; outer covering of plant
ground; the tissues that sopport the plant, carry out photosynthesis or store photosynthetic product
vascular; movement of water/solutes
discuss the stages plants go through when developing a body plan
1) a zygote divides into an apical daughter cell and a basal daughter cell
2) apical daughter cell divides laterally and horizontally into an embryo
3) the 3 tissue systems form by differentiation
4) apical meristem fevelop at bast of embryo; allows for root system to develop
name the shapes a plant embryo goes through
octant, globular, heart, torpedo, seedling
name the model plant organism, name which family of plants it belongs to and why it has been selected as a model organism
Arabidopsis thaliana; cabbage family (brassicaceae) short life cycle, small genome, produces lots of seeds
state what experiment have been done on Arabidopsis thaliana to study the sequence of events in embryogenesis
mutation of genes to establish what phenotype characteristic the mutation related too
how do plants grow
1) cell elongation; uptake water into vacuole and loosen cell walls by expansin action
2) cell division from meristems
discuss location of meristems
tip of roots, shoots and leaves used for growth of shoot root and leaves
auxillary meristems at nodes used to produce lateral buds
cylinderical lateral meristems in stem/roots which allow for secondary grwoth (thickening)
discuss the root cap and quiescent centre
the root cap is an area without meristems which exists to protect the meristems when roots are penetrating through soil. it has gravity perception
the quiescent centre is of very slow cell division which produces tissues needed for the root cap to elongate
which plant hormones can be related to growth
the auxins and cytokinins cause apical dominance which leads to upward growth
the gibberelins cause elongation of internodes
what are the features of a rosette, and why is a rosette like this, what types of rosette are there
a plant that is very low to the ground with radial symmetry
internodes fail to grow
spiral phylotaxy
oppositr phyllotaxy
what does phyllotaxy mean
arrangemment of leaves arounf the stem
how can you measure plant growth and what are the equations for this
RGR=LAR x NAR
relative growth rate, leaf area ratio, net assimilation rate
what is the equation to measure leaf area ratio
LAR= SLA x LMR
specific leaf area, leaf mass ratio
whare some factors that influence plant growth and therefore explain why plants grow at ifferent rates
rate of photosynthesis
water availability
nutrient availability
genetic factors
what causes differentiation into specialised cells
internal signals like hromones as well as external signals like light induce gene expression changes
if all cells have the same genetic mateiral then how do cells differentiate into different cells
different sets of genes are expressed
discuss different plant epridermal cells and their functions
pavement cells; morphologically unspecialised cells which place out stomata and have structural function
stomata; regulate water and gas exchange
trichomes; produce chemcials and therefore offer protection against predators
root hair cells; cells with increased surface area which are specialised for nutrient uptake
what is the function of cone shaped cells on the epidermis
enhance heat and light capture
which genes and hormone regulate senescence. which cell process is key
senescence associated genes (SAG’s)
ethylene
apoptosis
what is the function of the flower has a whole
provide a mechanism of sperm and egg union
what are the two names for fertilisation in plants
outcrossing
selfing
discuss the anatomy of a flower
stamen (male reproductive organs) a filament sopports an anther which produces pollen (contians sperm)
carpel (female reproductive organs); a sticky stigma traps pollen via its hairs
an ovary exists below the stigma which produces eggs; a style connects the two structures
petals; brightly coloured to attract pollinator animals
sepals; leafy parts that wrap around flower bud and protect it during development
denine pollination
movement of pollen (containing sperm) from the anther to the stigma
what percentage of plants are flowering plants
60%
what initiates flowering
a developmental switch causes a shift from development of vegetative organs to reproductive organs;
vegetative meristems stop producing leaves and reproductive meristems start divising to produce flower parts
what can signal for flowering to begin
vernalisation (coldness), day length (photoperiod) or developmental age
what plant was used to investigate photoperiod? what were the results
a mutant tobacco (maryland mammoth) and soy beans
even if flowers are sewn over a 3 month period they all still flower within a 3 week period in september
compare short day, long night plants against long day, short night plants,against day neutral plants
short night;
hanbane
only flower if day length is above 14 hours
flower in spring/early summer
long night;
maryland mammoth
day length has to be shorter than 14 hours
flowering ococurs in late late summer/autumn
day neutral;
maize, tomato
flowering is regulated by other factors apart from dya length, such as temperature and age
whis is key in photoperiodism initiating flowering, day length or night length
night length
what percieves photoperiod, meristems of leaves
leaves, masking leaves causes flowering changes whereas masking meristems does nothing
what is the molecule that absorbs red and far red light, how does it initiate flowering
phytochtome absorbs red/far red light via its chromaphore
the molecule undergoes a confirmational shape change which impacts gene expression by interacting with transcription factors
red light and far red light exist, which causes flowering and which causes dormancy
red light causes flowering
far red light causes dormancy
these different lights reverse the effectsof eachother; the last wavelength the plant is exposed to is the one that has its effect
discuss the GNR relating to photoperiod induced flowering
1) when the correct photoperiod is present, the CONSTANS protein is stabilised
2) transcription of florigen ococurs (flowering locus T)
3) florigen enters phloem, travels to leaf and combines with flowering locus D; trnascription of APTELA1
4) APTELA1 initates flowering by commiting meristems to produce floral genes not vegatatitve genes
organ identiy genes control development of floral parts
where is florigen synthesised
phloem companion cells before being translocated
what type of gene if FLOWERING LOCUS T
What type of gene is APTELA1
FT is a flowering time genes; determines when flowering is initiated
APTELA1 is a floral identity gene
what is the ABC model of plan development
the idea that plants have 4 whorls (parts); sepals, petals, stemens, carpels and each whorl requires a different set of genes to be expressed for normal development.
how has the ABC model of the 4 whorls been investigated, what were the results
using mutants; if the a gene set is mutated then all the neccassary flower parts dont develop
which gene sets are antagonistic (prevent expression of eachother)
gene set A (APTALA1, APTALA2) and set C (AGAMOUS)
draw the table of gene sets and which whorls require them
check in notes
more complicated way of saying 24 hours and how this related to plants
circadian time cycle
leaves and flowers move in a 24 hour cycle and gene expression changes throughout the course of the day
why is plants having the ability to measure time an advanatge
it means changes in environment can be anticipated
when does a stem growth occour
night time
is the circadian rhythm effected by temperature, does the rhythm persist in the absence of external cues, can the rhythm be reset by external cues
no
yes
yes
what piece of equipment is used to measure leaf movements
a rotating drum
what happens when bean plants are placed in constant light
they operate of a 25.7 hour cycle. amplitude of movements decreases
what picks up light signal
what does this molecule reset which untilamtely causes differing gene expression
phytochrome
resets central oscillator
CCA1 (an MYB family gene) is expressed early in the day which blocks expression of TOC1 gene.
at night TOC1 gene (MYB gene) is expressed which blocks expression of the CCA1 gene
discuss concentrations of Pr and Pfr over the course of the day/night
Pfr is converted to Pr over the course of the day, when is converted back to Pfr overnight
what are the two carbodydrate molecule in plants and where are they synthesised
starch; synthesised in chloroplast
sucrose; synthesises in cytosol
do most plants store starch or sucrose in the roots, what an example of a plant that doesnt
most store starch
beet stores sucrose
what molecule are carbodydrates moved as and why
starch; it is used for transport because its a stable non reducing sugar
what is evidence for the phloem being used to transport of assimilates
dridling (ring barking) blocks translocation
radioactive marked sucrose has been shown to be moed into the phloe
what species of plant has mainly amino acids in its phloem, compared to most plants (mainly sucrose in the phloen)
cubitacaea
discuss the anatomy of the phloem system
sieve tubes; nuclues lacking, organelle lacking tubes
sieve plates exist between sieve tube cells, they have holes in them to allow passage of assimilates
companion cells; interacts with sieve tube element and has a role with loading/unlaoding of assimilates
what is the bundle sheath
a layer of cells sorrounding the vascular bundle
what is the pressure flow model
a model which explains transport of assimilatesin the phloem;
when assimilates are loaded into the sieve tube, the water potential to decrease, so water moves by osmosis from xylem into this part of the phloem; hydrostatic increases and forces sap through sieve plates towords sink.
at sink the assimilates are unloaded, which increases water potantiol so water moves back into the xylem
is there a low or high energy requirement for translocation
low
what are the two methods of laoding assimilates into the phloem
symplastic; through plasmodesmata. requires a high concentration gradient
apoplastic; assimialtes are moved across cell membranes into apoplast. this requires energy
what is the polymer trapping model
the idea that the cells between the vascular bundle and the bundle sheath convert sucrose into raffinose (trisaccaride) and stachyose (tetrasaccaride) before the molecules enter the sieve tube
what are the 3 routes of unloading from the phloem; which plants use each method
symplatic; sucrose moves into cells via plasmodesmata;
leaves/root tips
apoplastic; sucrose is unloaded into apoplast before active transport into cells
sugar cane
apoplast; sucrose is unloaded into the apoplast before being hydrolysed into glucose and fructose which is then actively transported into cell
legumes
how is it determined which sink the assimilates go to
sink strength (sink size x sink activity) proximity of sink to source is important as well as the vascular connections to different sinks
discuss stomata physiology
2 guard cells exist which have thickened inner walls.
stomatal pore between guard cells
guard cells are sorrounded by subsiduary cells
discuss the three different ways stomata can be arranged
randomly
clustered
in linear rows
whats the equation for stomatal density
number of stomata/leaf area
what is the stomatal index
the number of stomata in relation to the number of epidermal cells
which gene controls stomatal pattrerning
ERECTA (ER)
discuss how monocot and dicot ( 2 types) differ in where stomata are located
monocot; 50% lower
woody dicot; 100% lower
non woody dicot; 60% lower
what is the name for a non woody dicot
herbaceous
discuss the mechanism by which stomata open
1) H+ expelled from guard cells and create an electrochemical gradient (ATPas activity)
2) K+, Cl- and H+ enter guard cells from subsiduary cells, and are transported to the vacuole reducing water potentiol
3) water enters which causes cells to swell
what is the mechanism by which stomata close
1) Ca+ enters guard cells
2) K+ and Cl- channels open causing these molecules to leave guard cell down thier concentration gradient
3) what potentiol in vacuole increases so water leaves
Ca+ also inhibits the action of the H+ ATPase