Deck 2 Flashcards
What is an operator?
It is the binding sites for repressors and activators
What is an operon?
It is a cluster of genes that are regulated at a common site and are transcribed as a single mRNA by a common promoter
What does a repressor do?
It binds to an operator, which blocks RNA polymerases access to the promoter
A repressor results in the decrease of the synthesis of what?
mRNA
Does a inducer or corepressor alter repressor conformation so it cannot bind DNA, and mRNA can be synthesized
Inducer
A activator binds to what and results in the increase of the synthesis of what?
Operator
mRNA
In Horizontal Gene Transfer, what it sensitive to extracellular DNases?
Transformation
(T/F) In HGT, the donor and recipient must be of the same species, and it only occurs in 2 of the 3 domains
False, the recipient and donor can be of different species and it occurs in all 3 domains
In HTG, what mechanism is regulated by phage?
Transduction
(T/F) We need both a positive and negative charge to achieve bacterial growth
True
Which antibiotic inhibits cell wall synthesis and why?
Penicillin
Blocks the transpeptidation step of cell wall synthesis
The direction of Hfr transfer depends on what two factors?
Orientation of integrated F factor
Integration site
(T/F) In Hfr the donor chromosomal DNA is transferred at a high frequency
True
(T/F) Complete F factors are rarely transfered
True
When does generalized transduction occur and what causes it?
During the lytic phase
Errors in packaging