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Histopath > Decalcification > Flashcards

Decalcification Flashcards

1
Q

Decalcification temperature

A

18-30degC

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2
Q

An excellent bone decalcifier for EM and enzyme histochemistry

A

EDTA

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3
Q

EDTA can inactivate__

A

Alkaline phosphatase
Remedy: add magnesium chloride

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4
Q

Most rapid method of decalcification due to electric current calcium is removed

A

Electrolytic method

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5
Q

Method of testing for the completeness of decalcification that uses calcium carbonate

A

Bubble test

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6
Q

Bubble test uses

A

Calcium carbonate

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7
Q

If after decalcification, the tissue is still hard use

A

Molliflex-perenyi’s fluid

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8
Q

(Double purposes)
i. Decalcifying agent
ii. Tissue softener

A

Perenyi’s fluid

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9
Q

Decalcifying acid used for surface decalcification of blocks

A

Hydrochloric acid

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10
Q
  • most rapid nitric acid containing decalcifying agent
A

Phloroglucin nitric acid

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11
Q

T/F
Osteoporotic specimen has low calcium content = shorter decalcification time

A

True

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12
Q

Duration of decalcification thus ion exchange resin

A

1-14 days

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13
Q

Methods to measure extent of Decalcification

A
  1. Physical/mechanical
  2. X-ray/radiologic
  3. Chemical
  4. Bubble Test
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14
Q
  • Not a reliable method although it easy to do
  • done by bending the tissue or by pricking the tissue with needle
A

Physical method/Mechanical

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15
Q

Most reliable and can detect even the smallest amount of calcium

A

X-ray/Radiologic Method

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16
Q
  • Carried out by adding calcium carbonate
  • method of testing for the completeness of decalcification that uses calcium carbonate
A

Bubble test

17
Q

Transfer 5ml of discarded fluid on a tube

A

Chemical Method/calcium oxalate test

18
Q

Recomended for decalcifying cartilage, research specimen, autopsy specimen and Bone marrow

A

Formic acid sodium citrate

19
Q

Weak and slow

A

TCA, Sulfurous acid

20
Q

Not commonly use because it is considered as environmental Toxin and its Highly Corrosive on the skin

A

Chromic acid /flemming’s with HAC

21
Q

Contains chloroform as preservatives

A

Citric acid citrate buffer

Histopath (3 decks)

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