Dario

Question 1

1 ITAM - thymic phenotype

Answer 1

Functional ITAMS: Gamma or delta

• No Thymic T cells (#)
• Thymocytes = 90% DN3

Question 2

1 ITAM implications

Answer 2

Need at least 2 ITAMs to support DN3-DN4 transition (pre-TCR).
gamma may be able to support expansion of CD4+ cells in periphery if you are able to generate them

Question 3

1 ITAM Splenic phenotype

Answer 3

Functional ITAMS: Gamma or delta

• gamma: A few T cells (lower than WT) mostly CD4+ (CD4/8=4) (40% of mice have detectable cells)
• delta: No splenic T cells

Question 4

CD5 levels and ITAM multiplicity

Answer 4

Lower # of ITAMS have higher expression of CD5 on SP compared to WT

Question 5

Relationship between ITAM# and proliferation

Answer 5

More ITAMS=Better proliferation
Linear regression, with the exception of mice with 1 ITAM (gamma or delta) Unique modulatory influence for these itams on T cell proliferation