Cytoprep and Cytology Staining Flashcards
What type of stain is the DiffQuik?
Romanowsky. It is a faster version of the Wright Giemsa, using 3 solutions: Sol1 methanol (often with Malachite Green); Sol2 xanthene dye (usually Eosin y); Sol3 methylene blue and Azure A
What is the main stain for cytology?
The Papanicolaou or Pap stain.
How many dyes are used in the Pap stain?
Five. Hematoxylin, Orange G, Eosin, Light green, and Bismark brown.
True or False: EA is a polychromatic dye.
True. It consist of Eosin, Light Green, and Bismarck Brown.
How is the Pap stain different from FFPE special stains?
The preferred fixative is alcohol, it was developed for demonstrating cells from body fluids rather than tissue, and it is meant to stain transparently.
What is the mlst common fixative group used in cytology?
Alcohols.
What fixative is used for upper respiratory tract cytology specimens?
Saccomanno Fluid.
What is a ThinPrep?
A common way to process cytology specimens.
What are some common fixatives used with the ThinPrep Instrument?
PreservCyt, UroCyte, CytoLyt.
Name some non-gynecologic cytology specimens.
Fine needle aspirates (FNAs), urine, sputum, cerebral spinal fluid (CSF).
What is the main difference between CytoRich Red and CytoLyt?
CytoRich Red contains formaldehyde.
Why is Carnoy’s solution used on bloody smears?
It hemolyzes red blood cells.
