Cytology Flashcards
1
Q
How does light microscope compare to electron to scanning?
thickness and resolution.
What is the thickness of a phospholipid bilayer?
A
LM: 5 - 10 micrometers
EM: 20-100 nanometer
Scanning EM (SEM) view surface of a solid specimen.
Resolution:
LM optical lenses focus visible light (200 nm)
EM: Resolution (.2-.5 nm)
Scanning EM resolution 3nm.
5-10 nm.
2
Q
What can you not see under a light microscope?
A
The double membrane. The nuclear pore.
Mitochondria.
3
Q
What are the thicknesses of the filaments?
A
Actin filaments (7nm)
Intermediate (10 nm)
Microtubules (25 nm)
