Cytogenetics And Molecular Genetics

Question 1

The long and short arms of chromosomes are designated as 
A. p and q arms
B. m and q arms
C. q and p arms
D. 1 and s arms

Answer 1

Answer - C. q and p arms

The short arm of chromosome is designated as p arm and the long arm is known as q arm.

Question 2

Karyotyping under light microscopy is
A. L banding
B. G banding 
C. C banding
D. FUdR banding

Answer 2

Answer- G banding

Question 3

Fluorescence means
A. Spontaneous illumination in dark
B. Release of longer wavelength light on absorbing light of shorter wavelength
C. Release of shorter wavelength light on absorbing light of longer wavelength
D. Release of equal wavelength light at constant rate.

Answer 3

Answer- B. Release of longer wavelength light on absorbing light of shorter wavelength

Question 4

DNA estimation can be done by 
A. Spirometer
B. Spectrophotometer
C. pH meter
D. Sphygmomanometer

Answer 4

Answer- B. Spectrophotometer

Quantitative estimation of DNA and RNA can be done by UV spectrophotometer at about 260nm and densitometry.

Question 5

True about restriction endonuclease function is
A. Cut both the strands of Ds DNA
B. The cut ends produced are sticky
C. The cut ends produced are blunt 
D. Cuts single strand of DNA

Answer 5

Answer- A. Cut both the strands of Ds DNA
B. The cut ends produced are sticky
C. The cut ends produced are blunt

Question 6

Rapid method of chromosome identification in intersex is 
A. FISH
B. PCR
C. SSCP
D. Karyotyping

Answer 6

Answer- A. FISH

FISH, Chromosome painting and special Karyotyping are rapid methods of chromosome identification.

Question 7

Purpose of gene therapy
A. Replacement of abnormal gene by normal gene
B. Replacement of normal gene by a normal gene
C. Knock out of abnormal gene
D. Introduction of viral gene

Answer 7

Answer- A.Replacement of abnormal gene by normal gene