Cuture Medium

Question 1

-Singular

-nutrient material prepared for the growth of microorganism in a laboratory

Answer 1

Culture Medium

Question 2

Plural of Culture Medium

Answer 2

Media

Question 3

Microorganism introduced into a culture medium to initiate growth

Answer 3

Inoculum

Question 4

microorganism that grow and multiply in and on a culture medium

Answer 4

Culture

Question 5

material devoid of any living microorganism and viruses

Answer 5

Sterile

Question 6

A complex polysaccharide and used as a solidifying agent added to the solid medium from marine alga. Solidifies at 40 degrees celcius

Answer 6

Agar

Question 7

process of destruction or removal of all forms of microorganisms

Answer 7

Sterilization

Question 8

material that a microrrganism feeds on

Answer 8

Substrate

Question 9

made up of proteins that catalyzes chemical reactions

Answer 9

Enzymes

Question 10

in test tube and petri dish

Answer 10

Solid Medium

Question 11

solid agar in test tubes

Answer 11

slants

Question 12

agar solidifies in a vertical tube

Answer 12

Deep

Question 13

Culture medium to solidify

Answer 13

Agar should be at 1.5-2%

Question 14

useful for identifying organisms based on colony characteristics

Answer 14

Solid media

Question 15

The bacterial population is considered derived from 1 bacterial cell

Answer 15

Colony

Question 16

Only in test tubes

Answer 16

Liquid broth

Question 17

measured by spectrophotometer called as optical density

Answer 17

Turbidity

Question 18

solidifying agent

Answer 18

Agar

Question 19

agar liquifies at what degree

Answer 19

100 degrees celcius

Question 20

gram-positive bateria mamebers of Genera Bacillus and Clostridium form vegetative cells and endospores

Answer 20

Spore Formers

Question 21

found in soil and endospores can be spread by the wind

Answer 21

Genera members

Question 22

common contaminants in the lab

Answer 22

Bacillus members

Question 23

to seal individual petri plates and ready for storage

Answer 23

Parafilm

Question 24

used to measure media powder; needed to calibrate first before using

Answer 24

Analytical Balance & Triple Beam Balance

Question 25

when to cover with aluminum foil

Answer 25

before autoclaving & after sterilization or storage

Question 26

used to transfer inocuum to test tubes and petri dishes; used in broths and solid medias

Answer 26

Inoculating Loop and Needle

Question 27

Stab Method of Inoculating Loop, explain

Answer 27

inoculating should be used in solid media with stab method

Question 28

sterilizes inoculating loop and needles; used in aseptic technique

Answer 28

Alcohol Lamp

Question 29

sterilizes inoculating loops and needles up to 10 seconds; uses 815 degrees celcius heat with infra-red heat

Answer 29

Bacti-cinerator sterilizer

Question 30

routine sterilization process for microbiology laboratory

Answer 30

Autoclaving

Question 31

specialized pressure cooker which uses moist heat; glasswares and small material instruments

Answer 31

Autoclave

Question 32

physical control of microbial growth; water vapor acids

Answer 32

Moist Heat

Question 33

maintains temperature settings, pour non-sterilized water inside, keeps media liquified

Answer 33

Water Bath

Question 34

melting & mixing flasks of media and liquids

Answer 34

Hot Plate

Question 35

to melt sterile media easier

Answer 35

Improvised Water Bath

Question 36

mixers of test tubes or conical centrifuge tubes to ensure solid particles into homogenous mixture

Answer 36

Vortex Mixer

Question 37

equipment that separates soilds, particles from liquid

Answer 37

Centrifuge

Question 38

color coded for different blood tests

Answer 38

Blood Collection Tubes

Question 39

uses dry heat and hot air which removes moisture

Answer 39

Oven

Question 40

maintains constant temperatrue; maintains with thermostat

Answer 40

Incubators

Question 41

stores microbial culture up to 2 years

Answer 41

Biological Refrigerators

Question 42

rotary shakes flasks

Answer 42

Shakers

Question 43

precision instruments that are provided with plastic tips fro dispensng liquid

Answer 43

Micropipettes

Question 44

transfers media to test tubes thru purshing button

Answer 44

Electronic Pipette Controller

Question 45

microbiologists use this instrument to measure turbidity, represented by optical density

Answer 45

Spectrophotometer

Question 46

avoids bubble formation while covering with cover slip

Answer 46

Glass Slides & Cover Slip

Question 47

used to tranfer sterile water to glass slides

Answer 47

Dropper & Glass Pasteur Pipettes

Question 48

separated solid particles with liquid

Answer 48

Filter paper

Question 49

to cultivate anaerobic microorganisms

Answer 49

Anaerobic Jar and Candle Jar

Question 50

prevents contamination and protects the operator from exposure from aerosol exposure to infectious agents

Answer 50

Biological Safety Cabinet (BSC)

Question 51

measurement of microbial growth

Answer 51

-total cell count -viable cell count or plate count -turbidity method

Question 52

covered with a cover slip, using micropipettor, dislodge a small drop of sample in a well in hemocytometer

Answer 52

Hemocytometer

Question 53

manually count colonies with a grid or computer assisted colony counter

Answer 53

Colony Counter Equipment