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Microbiology Laboratory: Introduction to Media & Equipment > Cuture Medium > Flashcards

Cuture Medium Flashcards

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Biology 101 flashcards
1
Q

-Singular

-nutrient material prepared for the growth of microorganism in a laboratory

A

Culture Medium

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2
Q

Plural of Culture Medium

A

Media

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3
Q

Microorganism introduced into a culture medium to initiate growth

A

Inoculum

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4
Q

microorganism that grow and multiply in and on a culture medium

A

Culture

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5
Q

material devoid of any living microorganism and viruses

A

Sterile

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6
Q

A complex polysaccharide and used as a solidifying agent added to the solid medium from marine alga. Solidifies at 40 degrees celcius

A

Agar

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7
Q

process of destruction or removal of all forms of microorganisms

A

Sterilization

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8
Q

material that a microrrganism feeds on

A

Substrate

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9
Q

made up of proteins that catalyzes chemical reactions

A

Enzymes

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10
Q

in test tube and petri dish

A

Solid Medium

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11
Q

solid agar in test tubes

A

slants

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12
Q

agar solidifies in a vertical tube

A

Deep

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13
Q

Culture medium to solidify

A

Agar should be at 1.5-2%

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14
Q

useful for identifying organisms based on colony characteristics

A

Solid media

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15
Q

The bacterial population is considered derived from 1 bacterial cell

A

Colony

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16
Q

Only in test tubes

A

Liquid broth

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17
Q

measured by spectrophotometer called as optical density

A

Turbidity

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18
Q

solidifying agent

A

Agar

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19
Q

agar liquifies at what degree

A

100 degrees celcius

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20
Q

gram-positive bateria mamebers of Genera Bacillus and Clostridium form vegetative cells and endospores

A

Spore Formers

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21
Q

found in soil and endospores can be spread by the wind

A

Genera members

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22
Q

common contaminants in the lab

A

Bacillus members

23
Q

to seal individual petri plates and ready for storage

A

Parafilm

24
Q

used to measure media powder; needed to calibrate first before using

A

Analytical Balance & Triple Beam Balance

25
Q

when to cover with aluminum foil

A

before autoclaving & after sterilization or storage

26
Q

used to transfer inocuum to test tubes and petri dishes; used in broths and solid medias

A

Inoculating Loop and Needle

27
Q

Stab Method of Inoculating Loop, explain

A

inoculating should be used in solid media with stab method

28
Q

sterilizes inoculating loop and needles; used in aseptic technique

A

Alcohol Lamp

29
Q

sterilizes inoculating loops and needles up to 10 seconds; uses 815 degrees celcius heat with infra-red heat

A

Bacti-cinerator sterilizer

30
Q

routine sterilization process for microbiology laboratory

A

Autoclaving

31
Q

specialized pressure cooker which uses moist heat; glasswares and small material instruments

A

Autoclave

32
Q

physical control of microbial growth; water vapor acids

A

Moist Heat

33
Q

maintains temperature settings, pour non-sterilized water inside, keeps media liquified

A

Water Bath

34
Q

melting & mixing flasks of media and liquids

A

Hot Plate

35
Q

to melt sterile media easier

A

Improvised Water Bath

36
Q

mixers of test tubes or conical centrifuge tubes to ensure solid particles into homogenous mixture

A

Vortex Mixer

37
Q

equipment that separates soilds, particles from liquid

A

Centrifuge

38
Q

color coded for different blood tests

A

Blood Collection Tubes

39
Q

uses dry heat and hot air which removes moisture

A

Oven

40
Q

maintains constant temperatrue; maintains with thermostat

A

Incubators

41
Q

stores microbial culture up to 2 years

A

Biological Refrigerators

42
Q

rotary shakes flasks

A

Shakers

43
Q

precision instruments that are provided with plastic tips fro dispensng liquid

A

Micropipettes

44
Q

transfers media to test tubes thru purshing button

A

Electronic Pipette Controller

45
Q

microbiologists use this instrument to measure turbidity, represented by optical density

A

Spectrophotometer

46
Q

avoids bubble formation while covering with cover slip

A

Glass Slides & Cover Slip

47
Q

used to tranfer sterile water to glass slides

A

Dropper & Glass Pasteur Pipettes

48
Q

separated solid particles with liquid

A

Filter paper

49
Q

to cultivate anaerobic microorganisms

A

Anaerobic Jar and Candle Jar

50
Q

prevents contamination and protects the operator from exposure from aerosol exposure to infectious agents

A

Biological Safety Cabinet (BSC)

51
Q

measurement of microbial growth

A

-total cell count
-viable cell count or plate count
-turbidity method

52
Q

covered with a cover slip, using micropipettor, dislodge a small drop of sample in a well in hemocytometer

A

Hemocytometer

53
Q

manually count colonies with a grid or computer assisted colony counter

A

Colony Counter Equipment

Microbiology Laboratory: Introduction to Media & Equipment (1 decks)

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