Cutaneous And Subcutaneous Mycoses Flashcards
Affect keratinized tissues
Cutaneous mycoses
Dermatophytes (keratinophilic)
Microsporon
Epidermophyton
Trichophyton
Causes ringworm/tinea
Dermatophytes (keratinophilic)
Ring-like appearance, red and scaly with a distinct margin, cordlike bumps beneath the skin fesembling a worm.
Tinea / Ringworm
Dermatophytes
Microsporum
Trichophyton
Epidermophyton
Microsporum infects
Skin, hair and rarely nails
Large, spindle pr cylinder shaped, thick walled, multi-septate, rough, spiny
Microsporum MACROconidia
Few or absent small, club shaped
Microsporum MICROconidia
Microsporum spp.
Microsporum audouinii
Microsporum canis
Microsporum gypseum
Anthrophilic
Microsporum auduoinii
Fluoresces under woods light (M. audouinii) color
Microsporum audouinii (yellow green)
Rice medium negative
Microsporum auduoinii
Salmon colored colonies
Microsporum audouinii
Fluoresces under woods light (M.canis) color
Bright yellow
Rice medium positive
Microsporum canis
Geophilic
Microsporum gypseum
No fluorescence
Microsporum gypseum
Rice medium positive
M. Canis
M. Gypseum
Cinnamon colored colonies
Microsporum gypseum
Trichophyton infects
Skin, hair, nails
Rare
Pencil shaped, multi-septate, thin walled, smooth borne singly on conidiophore
Tichophyton MACROconidia
Predominant
Spherical, tear shaped or clavate
Trichophyton MICROconidia
Trichophyton spp.
Trichophyton mentagrophytes
Trichophyton rubrum
Trichophyton tonsurans
Rose or epred-brown underside
Trichophyton mentagrophytes
Urease positive
Trichophyton mentagrophytes
Perforates hair
Trichophyton mentagrophytes
Star-shaped powder colonies
Trichophyton mentagrophytes
Microconidia in grapelike clusters
Trichophyton mentagrophytes
Deep cherry red or burgundy underside (enhanced on cornmeal or potato dextrose agar)
Trichophyton rubrum
Urease negative
Trichophyton rubrum
Does not perforate hair
Trichophyton rubrum
Tear shaped microconidia
Trichophyton rubrum
Tan or yellow rose with crater-like folds and wrinkled centers
Trichophyton tonsurans
Balloon shaped microconidia
Trichophyton tonsurans
Epidermophyton infects
Skin, nails, rarely hair
Club shaped, septate, thin-walled, smooth
Epidermophyton MACROconidia
Borne in slingles or cluster of 2-3 conidiophore
Epidermophyton MACROconidia
Absent
Epidermophyton MICROconidia
Epidermophyton spp.
Epidermophyton floccosum
Khaki colored colonies
Epidermophyton floccosum
affect deep layers of the skin, organisms found in soil thus feet usually affected, often result from a traumatic skin puncture from thorns or vegetation contaminated with fungi which are usually dematiaceous (darkly pigmented)
Subcutaneous mycoses
Fonsecaea pedrosoi
Fonsacaea compactum
Philaophora verrucosa
Cladosporium carrioniii
Exophiala jeanselmi
Exophioala spinifera
Wangiella dermatitidis
Chronic, non-healing, hard, warty, tumor-like lesions
Chromoblastomycosis
Characterized by cauliflower like lesions with SCLEROTIC BODIES” (copper-colored, septate cells)
Chromoblastomycosis
Chromoblastomycosis Characterized by cauliflower like lesions with
Sclerotic bodies
Chromoblastomycosis
Types of (Sporulations)
Acrotheca
Phialaphora
Cladosporium
Conidia in side
ACROTHECA:
Conidia in cluster
PHIALAPHORA:
CLADOSPORIUM
Conidia in chain
Mixed sporation
Fonsecaea
Phialaphora sporulation only
Phialaphora
cladosporium only Chromoblastomycosis
Cladosporium
Subcutaneous spp
Chromoblastomycosis
Phaeohyphomycosis
Mycetoma
Sporotrichosis or rose gardeners disease
Phaeohyphomycosis other term
Alternaria, Bipolaris, Curvularia, Dreschlera, Exophiala, Phialaphora, Wangiella
Opportunistic infections in subcutaneous
Phaeohyphomycosis
Chronic granulomatous infection of the cutaneous and subcutaneous tissue and bone
Mycetoma
Tumor-like deformities, abscesses, draining sinuses, granulomatous
Mycetoma
Actinomycotic mycetoma:
Nocardia, Actinomadura, Streptomyces
Eumycotic mycetoma:
Pseudoallescheria, Aspergillus, Exophiala, Acremonium, Curvularia, Madurella
Sporothrix or
Rose garderners disease
Sporotrichosis or Rose Gardener’s Disease
Caused by
Sporothrix schenkii
Dimorphic fungus in subcutaneous
Sporothrix schenkii
Sporotrichosis mold and yeast form
Mold form: narrow, septate hyphae with pyriform conidia arranged singly or in Flowerette arrangement
Yeast form: small, elliptoid budding, cigarette - shaped cells
Colonies become _______ (enhanced on potato dextrose or cornmeal agar), wrinkled and leathery with age
Black
Colonies become black (enhanced on potato dextrose or cornmeal agar), wrinkled and leathery with age
Sporotrichosis
Usually infects farmers or florists
Introduced by trauma, usually to hand
Sporotrichosis or Rose Gardener’s Disease
Types of tinea
Tinea capitis
Tinea barbae
Tinea corporis
Tinea crusis (jock itch)
Tinea Pedis (athletes foot
Tinea unguinum (Onchomycosis)
scalp, eyebrows, and eyelashes
Tinea capitis
smooth or glabrous skin (trunk)
Tinea corporis
beard
Tinea barbae
groin
Tinea Crusis (jock itch)
Foot
Tinea pedis
Nais
Tine unguium
affects the hair and skin
Microsporum
affects the skin and nails
Epidermophyton
affects the hair, skin, and nails
Trichophyton
Growth on rice medium
M. Canis
M. Gypseum
Woods lamp fluorescence
M. Canis
M.auduoinii
Abundant Red pigment
Trichophyton rubrum
Positive in hair baiting test
Trichophyton mentagrophytes (v shaped penetration)
“balloon-forms” aged microconidia; requires thiamine
Trichophyton tonsurans
Trichophyton schoenleinii:
favic chandelier; favus tinea capitis
Macroconidia: rare, 3-5 cells, thin- walled, “rat-tail”
Trichophyton verrucosum
Favic chandeliers and chlamydospores are common
Trichophyton schoenleinii
Swollen hyphae containing cytoplasmic granules
Trichophyton violaceum
What kind of fungus Sporotrichosis? Morphology in yeast and mold form
Dimorphic
rosette/flowerette/daisy petal conidia
cigar- shaped
Established infections appear as multiple, large, warty, <cauliflower-like= growths
Chromoblastomycosis
- Development of slow- growing verrucous nodules plaques
Chromoblastomycosis
What kind of mold chromoblastomycosis
Dermatiaceous mold
Chromoblastomycosis in tissue
In tissue: forms muriform cells (sclerotic bodies/Medler bodies) that are chestnut brown (copper pennies/black granule
formation of abscesses that contain large aggregates of fungal hyphae known as granules or grains
Mycetoma
Localized, chronic, granulomatous infections
Mycetoma
Fungus-Like Bacteria
Actinomycotic
composed of branching filaments, Gram-positive beaded rods
Granules (actinomycotic)
composed of septate fungal hyphae either hyaline or dematiaceous; PAS and GMS positive
Granules (eumycotic)
Subcutaneous Mucormycosis other name
Entomophthoromycosis
from traumatic implantation of the fungus present in plant debris
Subcutaneous Mucormycosis (Entomophthoromycosis)
Conidiobolus coronatus
(localized to the facial area mostly in adults)
Basidiobolus ranarum
(proximal limbs in children)
Hyphal fragments surrounded by intensely eosinophilic Splendore-Hoeppli material
Subcutaneous Mucormycosis (Entomophthoromycosis)
Hyphal fragments are thin walled and poorly staining.
Subcutaneous Mucormycosis (Entomophthoromycosis)
Require biopsy; histopathologic pictures are marked by focal clusters of inflammation with eosinophils and typical mucormycotic hyphae often surrounded
by eosinophilic Splendore-Hoeppli material.
Subcutaneous Mucormycosis (Entomophthoromycosis)
rare infection caused by dematiaceous saprobes
which invade organs (skin, lungs, brain) of immunosuppressed hosts
Phaeohyphomycosis
Phaeohyphomycosis
Cell wall pigmentation ranges from light to dark and may require special stains, such as the
Fontana-Masson melanin stain
Black molds in culture
Phaeohyphomycosis
Sporotrichosis causitive agent
Sporothrix schenckii
Chromoblastomycosis causative agent
Fonsecaea (mixed sporulation)
Cladosporium (only cladosporium sporulation) Exophiala Cladophialophora
Rhinocladiella
Phialophora (only phialophora sporulation)
Mycetoma causitive agent
Eumycotic
Exophiala
Phaeoacremonium
Curvularia
Fusarium
Madurella
Pyrenochaeta
Leptosphaeria
Scedosporium
Pseudallescheria boydii (most common)
Actinomycotic (Fungus-Like Bacteria)
Actinomyces
Nocardia
Known as true fungi
Eumycotic
most common mycetoma causative agent
Pseudallescheria boydii
Subcutaneous Mucormycosis (Entomophthoromycosis) causative agent
Conidiobolus coronatus
Basidiobolus ranarum
Phaeohyphomycosis causative agent
Exophiala jeanselmei
Wangiella dermatitidis
Bipolaris spp.
Alternaria spp.
Chaetomium spp.
Curvularia spp.
Phialophora spp. among others