Culturing Microorganisms (triple only) Flashcards

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1
Q

Name the process used by bacteria to reproduce

A

Binary fission

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2
Q

How frequently can bacteria reproduce given ideal contidionts?

A

Every 20 mintues

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3
Q

Name the two mediums that can be used in the lab to culture bacteria

A

Nutrient broth

Agar gel plate

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4
Q

Describe how an uncontaminated cuture of bacteria can be predpared using aseptic techniques

A

Sterilise petri dish

Sterilise culture media (agar gel or nurtient broth)

Sterilise inoculating loop by passing through a flame

Use inoculatinf loop to trasnfer micororganisms to the sterile medium

Secure the lid of Petri dish with adhesive tape

Store plate upside down

Incubate at 25oC

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5
Q

Why is the petir dish sealed with two pieces of adhesive tape?

A

Secures lid

Allows oxygen to diffuse inot the Petri dish

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6
Q

State the nutrients foudn in culture medium

A

Water

Glucose

Amino acids

Minerals

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7
Q

The picture shows an incubated pertir dish containing bacteria,.

What is the name given to the ‘dots’ of bacteria?

A

Colony

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8
Q

Why are school unable to incuabate bactrerial cultures at 37oC

A

Human pathogens would grow and increase in numbers

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9
Q

What is the name of the clear area around the filter paper discs on the plate?

A

Zone of inhibition

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10
Q

How could the area of the zone of inhibition labelled A be calculated?

A

Measure the diameter of the inhibition zone

Divide by 2 to get the radius

Use the equation

πr2

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