Culture Methods Flashcards
Enumerate types of culture methods
1.streak culture
2.lawn culture
3.stroke culture
4.stab culture
5.pour plate culture
6.liquid culture
What is the routinely used method to isolates bacteria?
Streak Culture
What is the purpose of using streak culture?
To obtain well separated colonies
(to obtain single colony)
Lawn culture also called as…….
Carpet culture
What is the purpose of using lawn culture?
Obtain uniform culture (carpet culture). So its useful for bacteriophage typing and antibiotic sensitivity testing. Also used for preparation of bacterial antigens and vaccines
What is the purpose of using stroke culture?
Providing a pure growth of the bacterium for slide agglutination and other diagnostic tests
What is the purpose of using stab culture?
Demostration of gelatin liquefaction and oxygen requirement of the bacterium under study. Also used in the maintenance of stock culture
What is the purpose of using pour plate culture?
Counting colonies of the bacteria
Creating anaerobic conditions called…..
Anaerobiosis
What is the 2 types of anaerobic jar?
1.MClntosh
2.Filde
Anaerobic jar is made up of either……… Or…..
Stainless steel or polycarbonate/polypropylene
Methods establishing anaerobic conditions in anaerobic jar are……..
- Anaerobic gas pak method
2.evacuation_replacement method
Gas pak is disposable packet containing pellets of…….
1.sodium borohydride
2.cobalt chloride
3. Citric acid
4.sodium bicarbonate
All gas paks need water to generate hydrogen and carbon dioxide. (T/F)
False. Some gaspak dont need water
Thioglycollate is one of anaerobic media. (T/F)
True
