Culture Media

Question 1

to maintain the viability of the organisms from source to the laboratory

Answer 1

Amie’s medium

Cary and Blair medium

Question 2

to increase the number of pathogenic vibrio in the specimen

Answer 2

Alkaline peptone water

Question 3

for isolation of all anaerobes

Answer 3

Anaerobic Blood agar

Question 4

for isolation of obligate Gram (+) and Gram (-) anaerobes. It inhibits growth of enteric bacilli and swarming of Proteus

Answer 4

Anaerobic Phenylethylalcohol (PEA) Agar

Question 5

For isolation of Gram (-) anaerobes especially Bacteroides, Prevotella and some Fusobacterium

Answer 5

Anaerobic Kanamycin Vancomycin Blood agar

Question 6

For isolation of Gram (-) anaerobes especially Bacteroides, Prevotella and some Fusobacterium. Detection of pigment production of prevotella

Answer 6

Anaerobic Paroromycin Vancomycin Laked Blood Agar

Question 7

Selective for Bacteroides sps. Used to differentiate bile resistant from bile sensitive Bacteroides sps.

Answer 7

Bacteroides Bile Esculin (BBE) medium

Question 8

Selective for group D Streptococcus and Enterococcus

Answer 8

Bile Esculin Medium (BEM)

Question 9

Selective for the isolation of Salmonella typhi

Answer 9

Bismuth Sulfite Agar (BSA) or Wilson Blair Agar

Question 10

Enriched with unheated blood. Differentiates organisms through hemolytic patterns.

Answer 10

Blood Agar

Question 11

Selective for the isolation of Bordetella pertussis

Answer 11

Bordet Gengou Agar or Potato Blood Glycerol Agar

Question 12

Basic medium for the isolation of microorganisms and can be used for preparing pure culture for antibiotic susceptibility test

Answer 12

Brain Heart Infusion (BHI) broth or agar

Question 13

For the isolation of Brucella spp.

Answer 13

Brucella Blood Agar

Biphasic media of Brucella Broth and Agar in Castaneda bottle or septi-chek

Question 14

for the isolation of Francisella tularensis

Answer 14

Blood Cystine Glucose Agar with Thiamine

Question 15

For the isolation of Legionella pneumophila

Answer 15

Buffered Charcoal Yeast Extract Agar (BCYE)

Question 16

For the isolation of Yersinia enterocolitica

Answer 16

Cefsulodin Irgasan Novobiocin (CIN) Agar

Question 17

For the isolation of Campylobacter spp.

Answer 17

Campy Blood Agar
Campylobacter Thioglycollate Broth
Cefoperazone, Vancomycin, Amphotericin medium

Question 18

Enriched with heated blood. For the cultivation of most organisms especially Haemophilus spp. and Neisseria spp.

Answer 18

Chocolate Agar

Question 19

For the isolation of Staphylococci

Answer 19

Chapman-Stone Agar

Question 20

For the isolation of Staphylococci and Streptococci from mixed specimen

Answer 20

Columbia Colistin Nalidixic Agar (CNA)

Question 21

For the isolation of anaerobes

Answer 21

Cooked meat or chopped meat medium

Question 22

For the isolation of Clostridium difficile

Answer 22

Cycloserine Cefoxitin Fructose Agar (CCFA

Question 23

For isolation and enumeration of bacteria in urine

Answer 23

Cystine Lactose Electrolyte Deficient (CLED) Agar

Question 24

For the isolation of Corynebacterium diphtheriae

Answer 24

Cystine Tellurite Blood Agar

Question 25

For the isolation of Clostridium perfringens

Answer 25

Clostrisel Agar

Question 26

For identification of Neisseria spp. based on their ability to utilize carbohydrate

Answer 26

Cystine Trypticase Agar (CTA)

Question 27

To identify Staphylococcus aureus based on its ability to produce DNASE enzyme

Answer 27

DNA agar

Question 28

To isolate organisms in CSF

Answer 28

Dextrose Ascitic Fluid Agar

Question 29

For the isolation of vibrio species

Answer 29

Dieudonne’s medium

Question 30

For the isolation of Mycoplasma and Ureaplasma spp.

Answer 30

“E” agar, Edward Hayflick Agar

Question 31

For the isolation of Haemophilus ducreyi

Answer 31

Fetal bovine serum agar with Vancomycin

Question 32

To identify Clostridium perfringens based on its ability to produce lecithinase

Answer 32

Egg Yolk Agar (EYA)

Question 33

Selective for Gram (-) bacilli. It differentiates lactose from non-lactose fermenting Gram (-) enteric bacilli

Answer 33

Eosin Methylene Blue Agar (EMB

Question 34

For the isolation of Leptospira interrogans

Answer 34

Ellinghausen, McCullough, Johnson and Harris (EMJH) medium;

Fletcher’s semisolid medium