culture media Flashcards
1
Q
Serial dilution method
A
- first take three 4-5 tubes
- add 1 ml solution in 9ml broth in the tube A,
- then A to B 1ml
- then B to C 1ml
- then C to D 1 ml
2
Q
Defined media
A
contain pure chemical substance. substance is exact concentration.
3
Q
Complex media
A
exact chemical composition is unknown.
4
Q
selective media
A
contain the substance which inhibit some organism and favor some
5
Q
differential media
A
color changes in the tubes
6
Q
microbial growth `(Lag, log, stationary, and death phase
A
- lag P. adapting new environment
- log p. growing
- stationary P. space is running out. toxin building
- death P. declining
7
Q
Cytometer
A
(cell meter) this determines the number size and nuclic acid contents of moving cell
8
Q
Spectrophotometer
A
a method to measure how much a chemical substance absorbs light by the intensity of the light beam.
