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MYCOLOGY AND VIROLOGY > CULTURE MEDIA > Flashcards

CULTURE MEDIA Flashcards

1
Q

What are the primary culture media used in mycology?

A

SABOURAUD DEXTROSE AGAR
BRAIN HEART INFUSION
SABOURAUD HEART INFUSION
INHIBITORY MOLD AGAR
POTATO DEXTROSE AGAR
POTATO FLAKE AGAR
V8 AGAR

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2
Q

What are the selective culture media used in mycology?

A

Mycosel/Mycobiotic Agar
Corn meal Agar w/ Tween 80
Czapek Agar
Niger Agar/Birdseed Agar
Cotton seed Agar

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3
Q

What are the selective/differential media?

A

Rice Extract Agar
Chrom Agar

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4
Q

What are the differential Media?

A

Trichophyton Agar
Urea Agar

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5
Q

For the Recovery of Pathogenic Fungi

A

SDA

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6
Q

what are the three organisms that are usually isolated in SDA without antibiotics

A

Aspergillus
C. neoformans
Candida

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7
Q

Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains

A

Brain Heart Infusion Agar

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8
Q

SDA+BHI=
(better combined)
Perfect for dimorphic fungi

A

SABOURAUDS HEART INFUSION AGAR

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9
Q

Primary Recovery of dimorphic pathogenic fungi

A

Inhibitory mold agar

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10
Q

what are the two organisms that will not be recovered in IMA

A

Saprophytic fungi & Dermatophytes

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11
Q

Stimulates sporulation and pigmentation

A

Potato Dextrose Agar

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12
Q

Lowers the pH by 3.5 in PDA

A

10% Tartaric Acid

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13
Q

Primary recovery of Saprophytic and Dimorphic Fungi (Fastidious and slow growing)

A

Potato Flake Agar

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14
Q

Recommended for the cultivation of yeasts and molds

A

V8 agar

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15
Q

4 compositions of V8 agar

A

Yeast extract
glucose
v8 juice
L-asparagine

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16
Q

A highly selective media for the isolation of pathogenic fungi for non-sterile materials also used for the recovery of dermatophytes

A

Mycosel Agar/Mycobiotic Agar

17
Q

what are the two antibiotics used in mycosel agar

A

Cycloheximide and Chloramphenicol

18
Q

this is used for the inducement of Chlamydospore formation of Candida albicans

A

Cornmeal Agar w/ tween 80

19
Q

two purposes of tween 80

A

growth of candida
reduce the solid portion of the cornmeal to grow the pseudohyphae

20
Q

for the routine cultivation fungi especially:
aspergillus
penicillium
non-sporulating molds

A

CZAPEK AGAR

21
Q

CZAPEK AGAR IS USED FOR THE ROUTINE CULTIVATION OF?

A

ASPERGILLUS
PENICILLIUM
NON-SPORULATING MOLDS

22
Q

a selective media used for the Identification of C. Neoformans

A

NIGER SEED/BIRDSEED AGAR

23
Q

NIGER SEED/BIRDSEED AGAR detects the enzyme?

A

Phenoloxidase

24
Q

Bird Seed Agar contains:

A

Caffeic Acid
creatinine (Enhance Melanin)
Glucose
Chloramphenicol

25
Q

Caffeic Acid + Phenoloxidase =

A

Melanin

26
Q

A selective media used for the conversion of Blastomyces dermatitidis from mold to yeast

A

Cottonseed Conversion Agar

27
Q

for the differentiation of yeasts by means of their typical chlamydospores and on the basis of micromorphological Criteria
useful in growing Candida spp.

A

Rice Extract Agar

28
Q

for the differentiation of clinically-significant Candida Species

A

CHROM agar media

29
Q

Give the color of the following in CHROM agar:
C. albicans
C. tropicalis
C. glabrata
C. krusei

A

aqua blue (C. albicans)
dark blue (C. tropicalis)
smooth dark pink (C. glabrata)
fluffy pink (C. krusei)

30
Q

It is a differential media but also selective because it only grows Trychophyton species

A

Trichophyton Agars

31
Q

What are the 4 trichophyton agars and their composition

A

1: vitamin free
2: Inositol
3: Inositol and Thiamine
4: Thiamine

32
Q

Demonstrates the enzyme UREASE

A

UREA AGAR

33
Q

UREA AGAR DETECTS?

A

C. neoformans
T. mentagrophytes vs T. rubrum
Trichosporon spp.

34
Q

Urease breaks down urea to:

A

Ammonia, H2O, CO2

MYCOLOGY AND VIROLOGY (1 decks)

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