CULTURE MEDIA Flashcards
What are the primary culture media used in mycology?
SABOURAUD DEXTROSE AGAR
BRAIN HEART INFUSION
SABOURAUD HEART INFUSION
INHIBITORY MOLD AGAR
POTATO DEXTROSE AGAR
POTATO FLAKE AGAR
V8 AGAR
What are the selective culture media used in mycology?
Mycosel/Mycobiotic Agar
Corn meal Agar w/ Tween 80
Czapek Agar
Niger Agar/Birdseed Agar
Cotton seed Agar
What are the selective/differential media?
Rice Extract Agar
Chrom Agar
What are the differential Media?
Trichophyton Agar
Urea Agar
For the Recovery of Pathogenic Fungi
SDA
what are the three organisms that are usually isolated in SDA without antibiotics
Aspergillus
C. neoformans
Candida
Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains
Brain Heart Infusion Agar
SDA+BHI=
(better combined)
Perfect for dimorphic fungi
SABOURAUDS HEART INFUSION AGAR
Primary Recovery of dimorphic pathogenic fungi
Inhibitory mold agar
what are the two organisms that will not be recovered in IMA
Saprophytic fungi & Dermatophytes
Stimulates sporulation and pigmentation
Potato Dextrose Agar
Lowers the pH by 3.5 in PDA
10% Tartaric Acid
Primary recovery of Saprophytic and Dimorphic Fungi (Fastidious and slow growing)
Potato Flake Agar
Recommended for the cultivation of yeasts and molds
V8 agar
4 compositions of V8 agar
Yeast extract
glucose
v8 juice
L-asparagine
A highly selective media for the isolation of pathogenic fungi for non-sterile materials also used for the recovery of dermatophytes
Mycosel Agar/Mycobiotic Agar
what are the two antibiotics used in mycosel agar
Cycloheximide and Chloramphenicol
this is used for the inducement of Chlamydospore formation of Candida albicans
Cornmeal Agar w/ tween 80
two purposes of tween 80
growth of candida
reduce the solid portion of the cornmeal to grow the pseudohyphae
for the routine cultivation fungi especially:
aspergillus
penicillium
non-sporulating molds
CZAPEK AGAR
CZAPEK AGAR IS USED FOR THE ROUTINE CULTIVATION OF?
ASPERGILLUS
PENICILLIUM
NON-SPORULATING MOLDS
a selective media used for the Identification of C. Neoformans
NIGER SEED/BIRDSEED AGAR
NIGER SEED/BIRDSEED AGAR detects the enzyme?
Phenoloxidase
Bird Seed Agar contains:
Caffeic Acid
creatinine (Enhance Melanin)
Glucose
Chloramphenicol
Caffeic Acid + Phenoloxidase =
Melanin
A selective media used for the conversion of Blastomyces dermatitidis from mold to yeast
Cottonseed Conversion Agar
for the differentiation of yeasts by means of their typical chlamydospores and on the basis of micromorphological Criteria
useful in growing Candida spp.
Rice Extract Agar
for the differentiation of clinically-significant Candida Species
CHROM agar media
Give the color of the following in CHROM agar:
C. albicans
C. tropicalis
C. glabrata
C. krusei
aqua blue (C. albicans)
dark blue (C. tropicalis)
smooth dark pink (C. glabrata)
fluffy pink (C. krusei)
It is a differential media but also selective because it only grows Trychophyton species
Trichophyton Agars
What are the 4 trichophyton agars and their composition
1: vitamin free
2: Inositol
3: Inositol and Thiamine
4: Thiamine
Demonstrates the enzyme UREASE
UREA AGAR
UREA AGAR DETECTS?
C. neoformans
T. mentagrophytes vs T. rubrum
Trichosporon spp.
Urease breaks down urea to:
Ammonia, H2O, CO2
