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culture media Flashcards

1
Q

Distinguish Escherichia coli from shigella spp.

A

acetate agar

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2
Q

Acetate agar results
E.coli (_______)
Shigella (_______)

A

Acetate agar results
E.coli (positive)
Shigella (Negative)

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3
Q

Acetate agar
Turns ______ when
positive; incubated
at __°C for __ days.

A

Turns blue when
positive; incubated
at 35°C for 4 days.

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4
Q

Enrichment for vibrio and aeromonas spp.

A

Alkaline peptone water

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5
Q

bacteria grown in akaline peptone water

A

Vibrio spp.

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6
Q

Alkaline peptone water
___ pH allows
_____ to thrive;
subculture to ____
agar within ___
hours.

A

High pH allows
vibrios to thrive;
subculture to TCBS
agar within 18
hours.

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7
Q

For isolating MTB

A

American Trudeau
Society Medium

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8
Q

bacteria grown in American Trudeau
Society Medium

A

MTB

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9
Q

American Trudeau
Society Medium

______ with ______ ;
inhibits
_________.

A

Egg-based with
malachite green;
inhibits
contaminants.

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10
Q

Selective for bacterioides fragilis group

A

Bacteroides Bile
Esculin Agar

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11
Q

Bacteria growth in Bacteroides Bile
Esculin Agar

A

Bacteriodes fragilis group

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12
Q

Bacteroides Bile
Esculin Agar

_______ of
_______ leads to
______ colonies;
inhibits most
______.

A

Hydrolysis of
esculin leads to
black colonies;
inhibits most
anaerobes.

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13
Q

For general anaerobic bacteria

A

blood agar

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14
Q

bacteria growth in blood agar

A

anaerobes like bacteriodes, clostridium

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15
Q

blood agar

________; can be
supplemented with
__________ like
_________.

A

Sheep blood; can be
supplemented with
antibiotics like
kanamycin.

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16
Q

Enrichment for
Bordetella pertussis

A

Bordet-Gengou
Blood Agar

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17
Q

Bacteria growth in Bordet-Gengou
Blood Agar

A

Bordetella pertussis

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18
Q

Bordet-Gengou
Blood Agar

Contains ______,
_______, and
_______ ; selectivity
_______ with
_________.

A

Contains blood,
potato infusion, and
glycerol; selectivity
increased with
antibiotics.

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19
Q

Enrichment for Legionella spp.

A

Buffered Charcoal
Yeast Extract Agar

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20
Q

bacteria growth in Buffered Charcoal
Yeast Extract Agar

A

Legionella spp.

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21
Q

Buffered Charcoal
Yeast Extract Agar

Contains activated
charcoal; supports
growth of
Francisella and
Nocardia.

A

Contains activated
______; supports
growth of
_______ and
________.

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22
Q

Selective for
Campylobacter spp.

A

Campylobacter
Blood Agar

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23
Q

bacteria growth in Campylobacter
Blood Agar

A

Campylobacter spp.

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24
Q

Campylobacter
Blood Agar

Contains _______
to _______ other
bacteria; _______ at
_______°C under
_________________.

A

Contains antibiotics
to inhibit other
bacteria; incubate at
42°C under
microaerophilic
conditions.

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25
Enrichment medium for Haemophilus and Neisseria
Chocolate Agar
26
bacteria growth in Chocolate Agar
Haemophilus influenzae, Neisseria spp.
27
Selective for Pseudomonas aeruginosa
Cetrimide Agar
27
bacteria growth in Cetrimide agar
Pseudomonas aeruginosa
27
Chocolate Agar ____________ factors for ______; useful for ________ pathogens.
Lysed blood supplies factors for growth; useful for respiratory pathogens.
28
Cetrimide agar Contains ________, __________ __________, and ___________ to enhance ________ production.
Contains cetrimide, magnesium chloride, and potassium sulfate to enhance pigment production.
29
Selective for Clostridioides difficile
Cycloserine Cefoxitin Fructose Agar
30
bacteria growth in Cycloserine Cefoxitin Fructose Agar
Clostridioides difficile
31
Cycloserine Cefoxitin Fructose Agar _______ colonies with ________ media change indicate ________ fermentation of ________.
Yellow colonies with yellow media change indicate positive fermentation of fructose.
32
Selective differential for Gram-negative bacilli
Eosin-Methylene Blue Agar (EMB)
33
bacteria growth in Eosin-Methylene Blue Agar (EMB)
E. coli, Enterobacter spp., etc.
34
Eosin-Methylene Blue Agar (EMB) _________ fermenters produce _______ colonies, _______ has ________.
Lactose fermenters produce colored colonies, E. coli has metallic sheen.
35
Selective differential for Salmonella and Shigella spp.
Hektoen Enteric Agar
36
bacteria growth in Hektoen Enteric Agar
Salmonella spp.
37
Hektoen Enteric Agar ________ turns _______ due to _____ production, _____ remains _______.
Salmonella turns black due to H₂S production, Shigella remains colorless.
38
Enrichment for Streptococcus agalactiae (Group B)
Lim Broth
39
bacteria growth in Lim Broth
Streptococcus agalactiae
40
Lim Broth _______ and _______ acid inhibit other ______; subculture to ______.
Colistin and nalidixic acid inhibit other flora; subculture to blood agar.
40
Selective for Mycobacterium spp.
Löwenstein-Jensen Medium
41
bacteria growth in Löwenstein-Jensen Medium
Mycobacterium tuberculosis
42
Löwenstein-Jensen Medium Contains _______; used for _______ growth of _______.
Contains malachite green; used for egg- based growth of M. tuberculosis.
43
Selective differential for Gram-negative bacilli
MacConkey Agar
44
Bacteria growth in MacConkey Agar
E. coli, Salmonella, Shigella
45
MacConkey Agar Differentiates _______ fermenters (______) from _____ (_______).
Differentiates lactose fermenters (pink) from non- fermenters (colorless).
46
Selective for Staphylococcus spp.
Mannitol Salt Agar
47
bacteria growth in Mannitol Salt Agar
Staphylococcus aureus
48
Mannitol Salt Agar High _____ content (_____) selects for __________; ______ turns the ______ due to _______ fermentation.
High salt content (7.5%) selects for staphylococci; S. aureus turns the medium yellow due to mannitol fermentation.
49
Selective for Mycobacterium spp.
Middlebrook 7H10 and 7H11 Agars
50
Bacteria growth in Middlebrook 7H10 and 7H11 Agars
Mycobacterium tuberculosis
51
Middlebrook 7H10 and 7H11 Agars _______ media for cultivating ______ strains like _________.
Defined media for cultivating drug- resistant strains like M. tuberculosis.
52
General use for antibiotic susceptibility testing
Mueller-Hinton Agar
53
bacteria growth in Mueller-Hinton Agar
Various bacteria
54
Mueller-Hinton Agar Supports ________ for ________________.
Supports standard disk diffusion for antimicrobial susceptibility testing.
55
Selective for Neisseria gonorrhoeae and Neisseria meningitidis
New York City Medium
56
bacteria growth in New York City Medium
Neisseria spp.
57
New York City Medium Contains _______ and _______ agents for ______.
Contains hemoglobin and antimicrobial agents for selectivity.
58
For cultivation of fungi
Sabouraud Dextrose Agar
59
bacteria growth in Sabouraud Dextrose Agar
Fungi (dermatophytes, yeasts)
60
Sabouraud Dextrose Agar ______ and high _______ concentration encourage _____ growth.
Low pH and high dextrose concentration encourage fungal growth.
61
Selective for Vibrio spp.
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)
62
bacteria growth in Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)
Vibrio cholerae (yellow), Vibrio parahaemolyticus (green)
63
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS) High ___ and _____ inhibit many bacteria; differentiates ______ fermenters.
High pH and bile salts inhibit many bacteria; differentiates sucrose fermenters.
64
Differentiates enteric bacteria based on sugar fermentation and H₂S production
Triple Sugar Iron Agar (TSI)
65
bacteria growth in Triple Sugar Iron Agar (TSI)
Salmonella, Shigella
66
Triple Sugar Iron Agar (TSI) ______ precipitate forms with ______ production; _____ slant indicates _____/______ fermentation.
Black precipitate forms with H₂S production; acid slant indicates lactose/sucrose fermentation.
67
Selective differential for enteric pathogens
Xylose Lysine Deoxycholate (XLD) Agar
68
bacteria growth in Xylose Lysine Deoxycholate (XLD) Agar
Salmonella (black), Shigella (red)
69
Xylose Lysine Deoxycholate (XLD) Agar ________ detects _____ production (_______); ______ bile salt content enhances ______ recovery.
Ferric ammonium citrate detects H₂S production (blackening); lower bile salt content enhances Shigella recovery.

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