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culture media Flashcards

1
Q

Distinguish Escherichia coli from shigella spp.

A

acetate agar

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2
Q

Acetate agar results
E.coli (_______)
Shigella (_______)

A

Acetate agar results
E.coli (positive)
Shigella (Negative)

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3
Q

Acetate agar
Turns ______ when
positive; incubated
at __°C for __ days.

A

Turns blue when
positive; incubated
at 35°C for 4 days.

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4
Q

Enrichment for vibrio and aeromonas spp.

A

Alkaline peptone water

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5
Q

bacteria grown in akaline peptone water

A

Vibrio spp.

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6
Q

Alkaline peptone water
___ pH allows
_____ to thrive;
subculture to ____
agar within ___
hours.

A

High pH allows
vibrios to thrive;
subculture to TCBS
agar within 18
hours.

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7
Q

For isolating MTB

A

American Trudeau
Society Medium

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8
Q

bacteria grown in American Trudeau
Society Medium

A

MTB

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9
Q

American Trudeau
Society Medium

______ with ______ ;
inhibits
_________.

A

Egg-based with
malachite green;
inhibits
contaminants.

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10
Q

Selective for bacterioides fragilis group

A

Bacteroides Bile
Esculin Agar

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11
Q

Bacteria growth in Bacteroides Bile
Esculin Agar

A

Bacteriodes fragilis group

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12
Q

Bacteroides Bile
Esculin Agar

_______ of
_______ leads to
______ colonies;
inhibits most
______.

A

Hydrolysis of
esculin leads to
black colonies;
inhibits most
anaerobes.

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13
Q

For general anaerobic bacteria

A

blood agar

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14
Q

bacteria growth in blood agar

A

anaerobes like bacteriodes, clostridium

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15
Q

blood agar

________; can be
supplemented with
__________ like
_________.

A

Sheep blood; can be
supplemented with
antibiotics like
kanamycin.

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16
Q

Enrichment for
Bordetella pertussis

A

Bordet-Gengou
Blood Agar

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17
Q

Bacteria growth in Bordet-Gengou
Blood Agar

A

Bordetella pertussis

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18
Q

Bordet-Gengou
Blood Agar

Contains ______,
_______, and
_______ ; selectivity
_______ with
_________.

A

Contains blood,
potato infusion, and
glycerol; selectivity
increased with
antibiotics.

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19
Q

Enrichment for Legionella spp.

A

Buffered Charcoal
Yeast Extract Agar

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20
Q

bacteria growth in Buffered Charcoal
Yeast Extract Agar

A

Legionella spp.

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21
Q

Buffered Charcoal
Yeast Extract Agar

Contains activated
charcoal; supports
growth of
Francisella and
Nocardia.

A

Contains activated
______; supports
growth of
_______ and
________.

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22
Q

Selective for
Campylobacter spp.

A

Campylobacter
Blood Agar

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23
Q

bacteria growth in Campylobacter
Blood Agar

A

Campylobacter spp.

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24
Q

Campylobacter
Blood Agar

Contains _______
to _______ other
bacteria; _______ at
_______°C under
_________________.

A

Contains antibiotics
to inhibit other
bacteria; incubate at
42°C under
microaerophilic
conditions.

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25
Q

Enrichment medium
for Haemophilus
and Neisseria

A

Chocolate Agar

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26
Q

bacteria growth in Chocolate Agar

A

Haemophilus
influenzae,
Neisseria spp.

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27
Q

Selective for
Pseudomonas
aeruginosa

A

Cetrimide Agar

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27
Q

bacteria growth in Cetrimide agar

A

Pseudomonas
aeruginosa

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27
Q

Chocolate Agar

____________ factors for
______; useful for
________
pathogens.

A

Lysed blood
supplies factors for
growth; useful for
respiratory
pathogens.

28
Q

Cetrimide agar

Contains ________,
__________
__________, and
___________ to
enhance ________
production.

A

Contains cetrimide,
magnesium
chloride, and
potassium sulfate to
enhance pigment
production.

29
Q

Selective for
Clostridioides
difficile

A

Cycloserine
Cefoxitin Fructose
Agar

30
Q

bacteria growth in Cycloserine
Cefoxitin Fructose Agar

A

Clostridioides
difficile

31
Q

Cycloserine
Cefoxitin Fructose
Agar

_______ colonies with
________ media
change indicate
________
fermentation of
________.

A

Yellow colonies with
yellow media
change indicate
positive
fermentation of
fructose.

32
Q

Selective differential
for Gram-negative
bacilli

A

Eosin-Methylene
Blue Agar (EMB)

33
Q

bacteria growth in Eosin-Methylene
Blue Agar (EMB)

A

E. coli, Enterobacter
spp., etc.

34
Q

Eosin-Methylene
Blue Agar (EMB)

_________ fermenters
produce _______
colonies, _______ has
________.

A

Lactose fermenters
produce colored
colonies, E. coli has
metallic sheen.

35
Q

Selective differential
for Salmonella and
Shigella spp.

A

Hektoen Enteric
Agar

36
Q

bacteria growth in Hektoen Enteric Agar

A

Salmonella spp.

37
Q

Hektoen Enteric Agar

________ turns
_______ due to _____
production, _____
remains _______.

A

Salmonella turns
black due to H₂S
production, Shigella
remains colorless.

38
Q

Enrichment for
Streptococcus
agalactiae (Group B)

A

Lim Broth

39
Q

bacteria growth in Lim Broth

A

Streptococcus
agalactiae

40
Q

Lim Broth

_______ and
_______ acid inhibit
other ______;
subculture to ______.

A

Colistin and
nalidixic acid inhibit
other flora;
subculture to blood
agar.

40
Q

Selective for
Mycobacterium spp.

A

Löwenstein-Jensen
Medium

41
Q

bacteria growth in Löwenstein-Jensen Medium

A

Mycobacterium
tuberculosis

42
Q

Löwenstein-Jensen
Medium

Contains _______; used for _______ growth of _______.

A

Contains malachite
green; used for egg-
based growth of M.
tuberculosis.

43
Q

Selective differential
for Gram-negative
bacilli

A

MacConkey Agar

44
Q

Bacteria growth in MacConkey Agar

A

E. coli, Salmonella,
Shigella

45
Q

MacConkey Agar

Differentiates
_______ fermenters
(______) from _____
(_______).

A

Differentiates
lactose fermenters
(pink) from non-
fermenters
(colorless).

46
Q

Selective for
Staphylococcus spp.

A

Mannitol Salt Agar

47
Q

bacteria growth in Mannitol Salt Agar

A

Staphylococcus
aureus

48
Q

Mannitol Salt Agar

High _____ content
(_____) selects for
__________; ______ turns the
______ due
to _______
fermentation.

A

High salt content
(7.5%) selects for
staphylococci; S.
aureus turns the
medium yellow due
to mannitol
fermentation.

49
Q

Selective for
Mycobacterium spp.

A

Middlebrook 7H10
and 7H11 Agars

50
Q

Bacteria growth in Middlebrook 7H10
and 7H11 Agars

A

Mycobacterium
tuberculosis

51
Q

Middlebrook 7H10
and 7H11 Agars

_______ media for
cultivating ______ strains like
_________.

A

Defined media for
cultivating drug-
resistant strains like
M. tuberculosis.

52
Q

General use for
antibiotic
susceptibility
testing

A

Mueller-Hinton Agar

53
Q

bacteria growth in Mueller-Hinton Agar

A

Various bacteria

54
Q

Mueller-Hinton Agar

Supports ________ for
________________.

A

Supports standard
disk diffusion for
antimicrobial
susceptibility
testing.

55
Q

Selective for
Neisseria
gonorrhoeae and
Neisseria
meningitidis

A

New York City
Medium

56
Q

bacteria growth in New York City Medium

A

Neisseria spp.

57
Q

New York City
Medium

Contains
_______ and
_______ agents
for ______.

A

Contains
hemoglobin and
antimicrobial agents
for selectivity.

58
Q

For cultivation of
fungi

A

Sabouraud Dextrose
Agar

59
Q

bacteria growth in Sabouraud Dextrose Agar

A

Fungi
(dermatophytes,
yeasts)

60
Q

Sabouraud Dextrose
Agar

______ and high
_______
concentration
encourage _____ growth.

A

Low pH and high
dextrose
concentration
encourage fungal
growth.

61
Q

Selective for Vibrio
spp.

A

Thiosulfate Citrate
Bile Salts Sucrose
Agar (TCBS)

62
Q

bacteria growth in Thiosulfate Citrate
Bile Salts Sucrose
Agar (TCBS)

A

Vibrio cholerae
(yellow), Vibrio
parahaemolyticus
(green)

63
Q

Thiosulfate Citrate
Bile Salts Sucrose
Agar (TCBS)

High ___ and _____ inhibit many
bacteria;
differentiates
______ fermenters.

A

High pH and bile
salts inhibit many
bacteria;
differentiates
sucrose fermenters.

64
Q

Differentiates
enteric bacteria
based on sugar
fermentation and
H₂S production

A

Triple Sugar Iron
Agar (TSI)

65
Q

bacteria growth in Triple Sugar Iron
Agar (TSI)

A

Salmonella, Shigella

66
Q

Triple Sugar Iron
Agar (TSI)

______ precipitate
forms with ______
production; _____
slant indicates
_____/______
fermentation.

A

Black precipitate
forms with H₂S
production; acid
slant indicates
lactose/sucrose
fermentation.

67
Q

Selective differential
for enteric
pathogens

A

Xylose Lysine
Deoxycholate (XLD)
Agar

68
Q

bacteria growth in Xylose Lysine
Deoxycholate (XLD)
Agar

A

Salmonella (black),
Shigella (red)

69
Q

Xylose Lysine
Deoxycholate (XLD)
Agar

________ detects _____
production
(_______); ______
bile salt content
enhances ______
recovery.

A

Ferric ammonium
citrate detects H₂S
production
(blackening); lower
bile salt content
enhances Shigella
recovery.

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