culture media

Question 1

are desined for primary isolation of a broad spectrum of microbes and contain a mixture of nutrients that support the growth of both pathogenic and non pathogenic organisms

example
peptone water
nutrient broth
nutrient agar

Answer 1

general purpose media

Question 2

contain complex organic substances such as blood, serum or special growth factors and are designed to increase the number of desired microorganism withoud stimulating the rest of bacterial population

Answer 2

enrichment media

Question 3

2 commonly enrichment media

Answer 3

blood agar
chocolate agar

Question 4

contains general nutrients with 5-10% blood added to a blood agar base

Answer 4

Blood agar

Question 5

Hemolytic patterns

Answer 5

beta hemolysis
alpha hemolysis
gamma hemolysis

Question 6

shows complete lysis of red blood cells resulting in complete clearing around the colonies

Answer 6

beta hemolysis

Question 6

shows incomplete lysis of red blood cells, producing a greenish discoloration of the blood agar around the colonies

Answer 6

alpha hemolysis

Question 7

shows no hemolysis, resulting no chsnge in the medium

Answer 7

gamma hemolysis

Question 8

a type of nurient medium that is used for the culture of fastdious organisms such as Haemophilus sp. Heat is applied to lyse the red blood cells, causing the medium to turn brown

Answer 8

chocolate agar

Question 9

contain one or more subsances that encourage the growth of only a specific target microorganism and inhibit the growth of others, ( prevent the growth of unwanted contaminating bacteria or commensals so only tha target bacteria will grow

Answer 9

selective media

Question 10

contains the antibiotics trimethroprim, nystatine, vancomycin and colistin, used for the isolation of neisseria

Answer 10

thayer-martin agar

Question 11

contains 10% NaCl and used for the isolation of staphylococcus aureus

Answer 11

mannitol salt sugar

Question 12

promotes the grwoth of gram negative bacteria, primarily those belongin to the famil Enterobacteriaceae, and inhibits the growth of gram positive bacteria through the addition of bile salts

Answer 12

MacConkey Agar

Question 13

a selective medium used to recover mycobacterium tuberculosis. It is made selective by the incorporation of malachite green

Answer 13

Lowenstein-Jensen medium

Question 14

used for the isolation of the fungi

Answer 14

saboraud dextrose agar

Question 15

allow the growth of several types of microorganism, design to show visible differences among certain groups of microorganism

example: macConkey agar, triple sugar iron agar

Answer 15

differential medium

Question 16

used for clinical specimens that need to be transported to the laboratory immediatley after collection, prevent drying of specimen and inhibit

Answer 16

transport media

Question 17

media used specifically for organism that cannot survive in the presence of oxygen and require reduced oxidation reduction potential and other nutrients

Answer 17

Anaerobic media