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MICRO/PARA > Culture Media > Flashcards

Culture Media Flashcards

1
Q

Non-fastidous organism, without giving growth advantage

A

Nutritive or supportive media

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2
Q

Nutrive/Supportive media

A

Tryptic soy agar or nutrient agar plates
Sabaroud dextrose agar
BAP and CAP

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3
Q

Required growth advantage of PARTICULAR pathogens

A

Enrichment media

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4
Q

Enrichment media

A

BCYE agar

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5
Q

Media for Legionella spp.

A

BCYE agar

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6
Q

supplemental or enrichment broth

A

Backup broth

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7
Q

Backup broth

A

Thioglycollate (thio) broth
Brain-heart infusion broth (BHIB)
Tryptic soy broth (TSB)
LIM broth
Gram NEG broth

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8
Q

One or more agents inhibit all organism except those “selected”

A

Selective media

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9
Q

Selective media

A

Phenylethyl alcohol (PEA)
Columbia agar with colistin and nalidixic acid (CNA)
MacConkey agar

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10
Q

Phenylethyl alcohol (PEA)

A

Gram POS bacteria

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11
Q

Columbia agar with colistin and nalidixic acid (CNA)

A

Gram POS bacteria

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12
Q

MacConkey agar

A

Gram NEG bacteria

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13
Q

Distinguish from other bacteria

A

Differential media

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14
Q

Differential Media

A

MacConkey agar
BAP

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15
Q

MacConkey agar

A

differentiate lactose fermenter and non lactose fermenter

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16
Q

BAP

A

determination by hemolytic patterns

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17
Q

Group D strep and enterococci

A

Bile Esculin Agar (BEA)

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18
Q

vancomycin-resistant Enterococci

A

Bile Esculin Azide agar with vancomycin

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19
Q

determination of hemolytic reactions

A

BAP

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20
Q

Bordetella pertussis

A

Bordet-Gengou agar

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21
Q

Legionella spp

A

Buffered charcoal yeast extract agar

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22
Q

enrichment and selection of Legionella spp

A

BCYE with antibiotics

23
Q

Campylobacter

A

Campy-blood agar
Campylobacter thioglycollate broth agar
Cefoperazone, vancomycin, amphotevicin (CVA) medium
Skirrow medium

24
Q

Cefsulodin-irgasan-novobiocin (CIN) agar

A

Yersinia spp
Aeromonas

25
Q

Chocolate agar

A

Haemophilus spp
Pathogenic Neisseria spp

26
Q

Columbia Colistin-Nalidixic acid agar

A

Gram POS bacteria

27
Q

Cystine tellurite blood agar

A

C. diptheriae

28
Q

Eosin Methylene blue (EMB) agar
MacConkey agar

A

Lactose fermenting vs nonlactose fermenting enteric bacilli

29
Q

Gram NEG broth

A

Enteric pathogens/gram NEG

30
Q

Hektoen Enteric agar

A

Salmonella and Shigella spp vs gram neg enteric bacilli

31
Q

MacConkey sorbitol agar

A

selection of E.coli 0157:H7 (colorless) non sorbitol fermenter

32
Q

Mannitol salt agar

A

Staphylococcus spp

33
Q

Mannitol fermentation, yellow colonies in MSA

A

S. aureus

34
Q

Not mannitol fermenter, colorless in MSA

A

S. epidermidis

35
Q

New York City agar

A

N. gonorrhoeae

36
Q

Phenylethyl agar

A

Gram POS cocci and anaerobic gram NEG bacilli

37
Q

Regan-Lowe agar

A

B. pertussis

38
Q

Salmonella Shigella agar

A

Salmonella and Shigella

39
Q

Schaedler agar

A

nonselective for recovery of anaerobes and aerobes

40
Q

Selenite broth

A

Salmonella

41
Q

Streptococcal selective agar (SSA)

A

S. pyogenes and S. agalactiae

42
Q

Tetrathionate broth

A

Salmonella and Shigella

43
Q

Thayer-martin agar

A

anaerobes, aerobes, microaerophilic and fastidious organism

44
Q

Thiosulfate citrate-bile salt (TCBS) agar

A

Vibrio spp

45
Q

Todd-Hewitt broth supplemented with antibiotics

A

S. agalactiae in female genital spx

46
Q

Trypticase soy broth

A

subculturing from primary agar plates

47
Q

Xylose Lysine Desoxychilate (XLD) agar

A

Salmonella and Shigella vs other gram neg bacilli

48
Q

Cycloserine cefoxitin fructose agar

A

Clostridium diFFicile

49
Q

Deoxycholate agar

A

Enterobacteriaceae

50
Q

Enterobacteriaceae

A

Deoxycholate agar
Xylose lysine deoxycholate agar

51
Q

Sheep BAP from wound culture

A

B-hemolytic strep and coagulase POS staph
Proteus and E. coli
Pseudomonas and Acinteboacter

52
Q

Prereduced and Vit K supplemented BAP

A

Bacteroides, Peptostreptococcus, Clostridium spp

53
Q

Spx for virus culture contain

A

Antibiotics and nutrient

54
Q

CSF spx transport

A

3%-7% CO2 at 35 C to 37 C

MICRO/PARA (5 decks)

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