Culture Media Flashcards by Yloisa Gurrea

Non-fastidous organism, without giving growth advantage

Nutritive or supportive media

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Nutrive/Supportive media

Tryptic soy agar or nutrient agar plates
Sabaroud dextrose agar
BAP and CAP

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Required growth advantage of PARTICULAR pathogens

Enrichment media

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Enrichment media

BCYE agar

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Media for Legionella spp.

BCYE agar

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supplemental or enrichment broth

Backup broth

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Backup broth

Thioglycollate (thio) broth
Brain-heart infusion broth (BHIB)
Tryptic soy broth (TSB)
LIM broth
Gram NEG broth

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One or more agents inhibit all organism except those “selected”

Selective media

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Selective media

Phenylethyl alcohol (PEA)
Columbia agar with colistin and nalidixic acid (CNA)
MacConkey agar

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Phenylethyl alcohol (PEA)

Gram POS bacteria

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Columbia agar with colistin and nalidixic acid (CNA)

Gram POS bacteria

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MacConkey agar

Gram NEG bacteria

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Distinguish from other bacteria

Differential media

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Differential Media

MacConkey agar
BAP

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MacConkey agar

differentiate lactose fermenter and non lactose fermenter

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BAP

determination by hemolytic patterns

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Group D strep and enterococci

Bile Esculin Agar (BEA)

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vancomycin-resistant Enterococci

Bile Esculin Azide agar with vancomycin

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determination of hemolytic reactions

BAP

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Bordetella pertussis

Bordet-Gengou agar

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Legionella spp

Buffered charcoal yeast extract agar

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enrichment and selection of Legionella spp

BCYE with antibiotics

Campylobacter

Campy-blood agar
Campylobacter thioglycollate broth agar
Cefoperazone, vancomycin, amphotevicin (CVA) medium
Skirrow medium

Cefsulodin-irgasan-novobiocin (CIN) agar

Yersinia spp
Aeromonas

Chocolate agar

Haemophilus spp Pathogenic Neisseria spp

Columbia Colistin-Nalidixic acid agar

Gram POS bacteria

Cystine tellurite blood agar

C. diptheriae

Eosin Methylene blue (EMB) agar MacConkey agar

Lactose fermenting vs nonlactose fermenting enteric bacilli

Gram NEG broth

Enteric pathogens/gram NEG

Hektoen Enteric agar

Salmonella and Shigella spp vs gram neg enteric bacilli

MacConkey sorbitol agar

selection of E.coli 0157:H7 (colorless) non sorbitol fermenter

Mannitol salt agar

Staphylococcus spp

Mannitol fermentation, yellow colonies in MSA

S. aureus

Not mannitol fermenter, colorless in MSA

S. epidermidis

New York City agar

N. gonorrhoeae

Phenylethyl agar

Gram POS cocci and anaerobic gram NEG bacilli

Regan-Lowe agar

B. pertussis

Salmonella Shigella agar

Salmonella and Shigella

Schaedler agar

nonselective for recovery of anaerobes and aerobes

Selenite broth

Salmonella

Streptococcal selective agar (SSA)

S. pyogenes and S. agalactiae

Tetrathionate broth

Salmonella and Shigella

Thayer-martin agar

anaerobes, aerobes, microaerophilic and fastidious organism

Thiosulfate citrate-bile salt (TCBS) agar

Vibrio spp

Todd-Hewitt broth supplemented with antibiotics

S. agalactiae in female genital spx

Trypticase soy broth

subculturing from primary agar plates

Xylose Lysine Desoxychilate (XLD) agar

Salmonella and Shigella vs other gram neg bacilli

Cycloserine cefoxitin fructose agar

Clostridium diFFicile

Deoxycholate agar

Enterobacteriaceae

Deoxycholate agar Xylose lysine deoxycholate agar

Sheep BAP from wound culture

B-hemolytic strep and coagulase POS staph Proteus and E. coli Pseudomonas and Acinteboacter

Prereduced and Vit K supplemented BAP

Bacteroides, Peptostreptococcus, Clostridium spp

Spx for virus culture contain

Antibiotics and nutrient

CSF spx transport

3%-7% CO2 at 35 C to 37 C