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Culture Flashcards

1
Q

Most commonly used solidifying agent

A

Agar

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2
Q

What is agar

A

Golden - Yellow granular powder

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3
Q

Agar is prepared from

A

seaweeds

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4
Q

Agar melts at

A

98 C

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5
Q

Agar sets at

A

42 C

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6
Q

Semi-solid agar concentration

A

0.2 - 0.5%

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7
Q

What is semi-solid agar used in demonstration of

A

bacterial motility and separating motile from non-motile strains

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8
Q

Semi-solid concentration are used in cultivating

A

Microaerophilic bacteria

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9
Q

Examples of Semi-solid media

A

Hugh and Leifson oxidation fermentation medium

Stuart’s and amies media

Mannitol motility media

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10
Q

What is liquid media referred to as

A

Broth

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11
Q

Bacteria grows uniformly producing general ___

A

Turbidity

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12
Q

Examples of liquid media

A

Nutrient broth

Peptone solution

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13
Q

Anaerobic media

A

Cultivates anaerobic bacteria at low oxygen

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14
Q

Extra nutrients found in anaerobic bacteria

A

Vit K, Hemin, O2

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15
Q

What is added in anaerobic media

A

glucose (1%), thiogycollate (0.1%), ascorbic acid (0.1%), cysteine (0.05%)

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16
Q

What fillings are part of anaerobic media

A

iron fillings

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17
Q

Examples of Anaerobic media

A

Robertson’s cooked meat medium

Thioglycolate broth medium

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18
Q

Where are aerobic media kept

A

Incubator

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19
Q

Examples of Aerobic media

A

Liquid media

Solid media

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20
Q

Peptone water composition

A

1%peptone + 0.5% Nacl +100ml water.

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21
Q

Nutrient agar composition

A

Nutrient broth + 2% agar

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22
Q

a container into which a lit candle is introduced before sealing the container’s airtight lid.

A

Candle Jar

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23
Q

What is suitable for Candle Jar

A

Microaerophile (capnophile) microorganisms

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24
Q

What is simple media primarily used for

A

primary used for the isolation of microorganisms

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25
Other name for simple media
Basal media
26
Simple purpose general media that
supports growth of non-fastidious microbes
27
Example of simple media
Nutrient broth Nutrient agar
28
such as blood agar, it has ingredients that exact components are difficult to estimate.
Complex media
29
specially prepared media from pure chemical substances for research purpose and composition of every component is well known
Synthetic or defined media
30
peptone water composition
1% peptone + 0.5% NaCl in water.
31
Substances like blood, serum, egg are added to the simple medium.
Enriched media
32
What is enriched media used for
For fastidious microorganisms in Neisseria gonorrhoeae
33
Examples of Enriched medium
Blood agar Chocolate agar
34
What mammalian blood is used for blood agar
sheep or horse
35
What concentration of blood is present in blood agar
5-10%
36
contain red blood cells that have been lysed by slowly heating to 80C
Chocolate agar
37
What is chocolate agar used for
Haemophilus influenzae
38
Beta hemolysis
Shows complete lysis of red blood cells resulting in complete clearing around the colonies
39
Alpha hemolysis
Shows incomplete lysis of red blood cells, producing a greenish discoloration of the blood agar around the colonies
40
Gamma hemolysis
No hemolysis resulting in no change in the medium
41
Selective media contains
Antibiotics Dyes Chemicals Alternation of pH
42
Thayer Martin medium selective is used for
Neisseria gonorrhoeae
43
What antibiotics are found in Thayer Martin Medium selective
- Vancomycin - Colistin - Nystatin - Trimethoprim
44
which is able to kill most Gram-positive organisms.
Vancomycin
45
which is added to kill most Gram-negative organisms except Neisseria.
Colistin
46
which can kill most fungi
Nystatin
47
which inhibits Gram-negative organisms, especially swarming Proteus.
Trimethoprim
48
The dye methylene blue in the medium inhibit the growth of gram positive bacteria.
Eosin methylene blue (EMB)
49
Is used for isolation of Campylobacter jejuni from fecal or rectal swab.
Campylobacter agar
50
Campylobacter agar contains
- Bacteriological charcoal, - Cefoperazone - Amphotericin B
51
contain penicillin, nalidixic acid and malachite green to inhibit growth of gram positive and gram negative bacteria, in order to limit growth to Mycobacteria species only.
Lowenstein -Jenson medium
52
Lowenstein -Jenson medium contains
penicillin, nalidixic acid malachite green
53
What grows in Lowenstein-Jenson medium
Mycobacterium tuberculosis
54
are designed in such a way that different bacteria can be recognized on the basis of their colony color.
Differential media
55
are incorporated so that those bacteria that utilize them appear as differently colored colonies.
Dyes and metabolic substrates
56
Distinguish between lactose fermenters & non lactose fermenters.
MacConkey medium
57
MacConkey medium distinguishes
lactose fermenters and Nonlactose fermenters
58
Color of lactose fermenters
Pink colonies
59
Color of nonlactose fermenters
colorless colonies
60
Mannitol fermenters includes
Staphylococcus aureus
61
Non-mannitol fermenters includes:
Staphylococcus epidermidis
62
Positive growth but non-mannitol fermenters includes:
Micrococcus luteus
63
Negative growth includes in Mannitol Salt Agar
- Escherichia coli, - Pseudomonas aeruginosa
64
Used for the isolation of Salmonella and Shigella spp from clinical enocimens and fond samples.
Xylose Lysine Deoxycholate Agar(XLD)
65
For cultivation of pathogen from urine specimen, inhibit swarming of proteus sp.
Cysteine Lactose Electrolyte Deficient
66
highly selective for the isolation of V. cholerae and V. parahaemolyticus
Thiosulfate-citrate-bile salts-sucrose
67
Delicate organisms may not survive the time taken for transporting the specimen without
Transport media
68
Examples of Transport media
- Stuart's medium - Buffered glycerol saline
69
Growth of organisms
Culture
70
enables growth inside of the body
Vivo
71
enables growth outside the body
Vitro
72
Turbidity standard
McFarland standard

MicroPara Lab (2 decks)

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