Cultivation

Question 1

facultative anaerobes

Answer 1

O2 is needed, but can ferment when must

Question 2

aerotolerant anaerobes

Answer 2

fermentation, but can tolerate the presence of O2

Question 3

microaerophils

Answer 3

(needs decreased O2, and increased CO2 concentration

Question 4

capnophils

Answer 4

(needs increased CO2 concentration

Question 5

Different Methods to do Anaerobic culturing.

Answer 5

anaerostat (closed chamber, filled with N2-CO2 mixture)
* Gas-Pack jar (closed chamber, sodium-bicarbonate salts produce CO2 and water)
* anaerobic incubator (sealed thermostat filled with CO2)
* certain liquid media (O2 is removed via boiling before culturing, inoculation and
sealing)
* adding chemical substances may help (thioglycolate)

Question 6

Generation time for colonies is

Answer 6

20mins

Question 7

colonies visible time is

Answer 7

24 hours

Question 8

tell ?me abt semi solid media in clturing of bacteria

Answer 8

0,7% agar concentration)

Question 9

enrichment media?

Answer 9

media used to allow faster growth of certain species than others due to special nutrient content

Question 10

special media?

Answer 10

• media to culture particular pathogens only

Question 11

selective media

Answer 11

only certain bacteria are able to grow due to antibiotic or dye supplementation

Question 12

BOUILLON WITH MEAT method is used for cultivation of which bacteria ?

Answer 12

Clostridia

Question 13

breaks down the meat
produces gas
(paraffin seal is pushed up is done by which bacteria?

Answer 13

Clostridium perfringes

Question 14

does not break down the meat

Answer 14

clostridium tetani