CT Impt Pointers Flashcards
How do the various molecular techniques work?
Pls revise mechanism of PCR etc..
What is TA cloning?
The T ends bind to the complementary A ends of PCR products amplified using Taq Polymerase..
Is there a need to know the names of restriction enzymes and plasmids?
No :) We do not mean to kill your brain cells 😂
What are the key features of a plasmid?
Polylinker, antibiotic resistance gene and Ori
What is Ori?
Ori is the start point of replication, allowing the plasmid to be replicated into host. Importantly, the plasmid can then survive in the bacterial host cell.
What are some important slides in Lecture 4A?
Slides 29, 30 and 32 are important.
No need to remember slides 26-28 and 33-34
What is the splicing mechanism?
Pls know the splice sites and exact mechanism of spliceosome-mediated RNA splicing
What is important from your E-learning?
It’s essential to know slide 6 :)
Is there a need to remember the specific sequences of SD and Kozak?
No, but pls know the names: ‘Shine-Dalgarno’ and ‘Kozak’ as well as their functions
What do we need to know about lac operon?
Everything! According to Dr Choy.. 😂
What are the key features for total RNA analysis?
Prokaryotes: 23S, 16S, 5S
Eukaryotes: 28S, 18S, 5.8S, 5S
Why should we look out for those distinct bands of 23S and 16S?
The distinct bands would serve as an indication of good RNA quality yielded
What is the prokaryotic transcription process?
Initiation (-35 and -10 Pribnow box form the promoter), elongation and termination..
What does the promoter consist of?
It is a DNA sequence
What is the function of Rho?
Binds to mRNA and dissociates it..
