cryotomie Flashcards by Anabelle Laplante | Brainscape

Q

pourquoi congeler

A

plus rapide
biopsie urgente
mise en evidence des lipides
fluorescence

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

angle du couteau cryostat

A

30 degré

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

quelle sorte de plaque sur le cryostat

A

antio roulement

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

nomme les 2 methodes de congelation

A

azote liquide
OCT

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

desavantage azote liquide

A

tissus mous ont tendance a craquer

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

quarrive il lors de congelation lente

A

formation de cristaux

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

quarrive il lors de congelation rapides

A

craquage de tissus mou

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

quarrive il quand le tissu est trop froid

A

tissu se degrade pendant la coupe

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

quarrive il quand la temperature est trop chaude

A

le tissu saccumule sur le bord de la lame

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

temperature interne du cryostat

A

-20C

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

comment on desinfecte le cryostat

A

vapeurs de formol
phenol 5%
alcool

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

comment on maintien le cryostat

A

decongele
nettoyer
lubrifier les parties mecaniques

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

que fait on si le mecanisme devien raide

A

traiter avec alcool 100%, secher et lubrifier

How well did you know this?

1

Not at all

2

3

4

5

Perfectly