crhomatograhy

Question 1

chromatography

Answer 1

separate molecules based on chemical or physical properties (mass, charge, hydrophobicity)

Question 2

Stationary phase

Answer 2

the molecule does not move with the sample and stays put

Question 3

mobile phase

Answer 3

the molecule moves with the sample

Question 4

paper chromatography

Answer 4

the stationary phase is a piece of filter paper, and the mobile phase travels through by capillary action

Question 5

TLC

Answer 5

stationary phase is a thin layer of inert material that coats the surface of a glass or plastic plate, placed into a closed take with enough eluting solvent, and capillary action draws the solvent up

Question 6

Column chromatography

Answer 6

the stationary phase consists of bead-like matrix that it’s a tube

Question 7

gradients

Answer 7

mobile phases of increasingly harsh character are used

Question 8

gel-filtration chromatography separates based on…

Answer 8

molecular mass

Question 9

Stokes radius (gel-filtration)

Answer 9

effective radius a molecule has as it tumbles rapidly in solution

Question 10

What size molecules elute first in gel-filtration?

Answer 10

large

Question 11

affinity chromatography

Answer 11

used for purification of specific biological macromolecules like proteins and nucleic acids

Question 12

affinity chromatography selectivity

Answer 12

it has the highest out of any chromatography

Question 13

antigen-antibody chromatography

Answer 13

takes advantage of great specificity and avidity of antigen-antibody interactions