Creatine Phosphate/Kinase Flashcards
How long can we sustain a contraction for in our muscles based purely on the amount of ATP present?
About 1 second
How can we get ATP from Creatine Phosphate?
Creatine Kinase converts creatine phosphate into creatine and ATP. Creatine phosphate acts as a buffer for demands for phosphate.
In what cells is CK in particularly high concentrations?
Creatine kinase (CK) is probably present in all cells but is present in particularly high concentrations in muscle cells and brain cells.
When cells rich in CK die, where does the CK go? Why is this relevant?
It is released into the circulation, therefore we can use it as a marker for cell damage.
Why do brain and muscle cells need a lot of ATP?
Brain and muscle cells need lots of ATP as they use a lot of energy and in quick bursts and so ATP is needed to quickly restore ion concentrations/equilibrium.
What are the three isoenzymes of CK called?
Three dimeric isoenzymes of creatine kinase are known in humans. The two different subunits M and B provide the following isoenzymes: MM, MB and BB.
Where is MB found?
Only in the heart, forming about 15% of it. The rest is all MM.
Where is BB NOT found?
The heart.
How can we separate the three isoenzymes of CK?
Electrophoresis
Which isoenzyme of CK moves the furthest towards the negative electrode?
MM
