Crash course Flashcards
What is the fundamental difference between viruses and bacteria?
Viruses are obligate intracellular parasites that lack cellular structure and metabolism; bacteria are cellular organisms with independent metabolism.
What are the essential components of a virus?
Nucleic acid (DNA or RNA) and a protein coat (capsid); some viruses also have an envelope.
How do viruses differ in their genetic material?
Viruses can have either DNA or RNA, which can be single-stranded (ss) or double-stranded (ds), and may be linear or circular.
What are the two main types of viral symmetry?
Icosahedral (spherical) and helical (rod-like).
What is the difference between an enveloped and a non-enveloped virus?
Enveloped viruses have a lipid bilayer from the host membrane; non-enveloped viruses lack this, making them more resistant to environmental stress.
What are the two major ways viruses are classified?
Baltimore Classification (based on nucleic acid type and replication strategy) and ICTV Classification (based on order, family, genus, species).
What are the seven Baltimore Classification groups?
I: dsDNA (e.g., Herpesvirus)
II: ssDNA (e.g., Parvovirus)
III: dsRNA (e.g., Reovirus)
IV: (+) ssRNA (e.g., Picornavirus)
V: (-) ssRNA (e.g., Orthomyxovirus)
VI: ssRNA with reverse transcriptase (e.g., Retrovirus)
VII: dsDNA with reverse transcriptase (e.g., Hepadnavirus)
What are the main steps of the viral replication cycle?
Attachment, Penetration, Uncoating, Replication, Assembly, Release.
What are the two main mechanisms of viral entry?
Endocytosis (clathrin-mediated or caveolin-mediated) and membrane fusion.
Which viruses use reverse transcriptase?
Retroviruses (HIV) and Hepadnaviruses (HBV).
What are the major outcomes of viral infection in a host cell?
Lytic infection: Cell destruction
Persistent infection: Long-term viral presence
Latent infection: Dormant virus with possible reactivation
Oncogenic transformation: Virus-induced cancer (e.g., HPV, EBV)
What are common viral cytopathic effects (CPEs)?
Cell rounding, syncytia formation (fusion), inclusion bodies, apoptosis.
What is the primary innate immune defense against viruses?
Interferons (IFN-α, IFN-β), NK cells, macrophages.
Which immune cells are crucial in controlling viral infections?
CD8+ T cells (cytotoxic T lymphocytes, CTLs).
What are the two types of viral vaccines?
Live attenuated (e.g., MMR, polio [Sabin])
Inactivated/killed (e.g., HepA, polio [Salk])
What is the genetic material of Influenza virus?
(-) ssRNA, segmented.
What is antigenic shift vs. antigenic drift in Influenza?
Antigenic drift: Minor mutations → seasonal flu
Antigenic shift: Major reassortment → pandemics
What is the receptor for HIV entry into cells?
CD4 (primary) and co-receptors CCR5/CXCR4.
What is the cause of mononucleosis?
Epstein-Barr virus (EBV).
What is the leading viral cause of congenital infections?
Cytomegalovirus (CMV).
What type of genome does Hepatitis B virus (HBV) have?
Partially double-stranded DNA with reverse transcription.
What is the most common cause of viral gastroenteritis?
Norovirus.
What is the gold standard for diagnosing viral infections?
PCR (Polymerase Chain Reaction).
Which test is commonly used for HIV diagnosis?
ELISA followed by Western Blot or PCR.