Cpac 1

Question 1

How is a control set up in a practical measuring enzyme activity

Answer 1

Replace the enzyme solution with distilled water or boiled enzyme solution

Question 2

How can the results of the practical measuring enzyme activity be used to find the initial rate of reaction

Answer 2

Plot your results on a graph of ‘rate of reaction against ‘time’ and draw a tangent at time =0 to find the initial rate

Question 3

Outline the practical procedure used to measure the effect of temperature on enzyme activity

Answer 3

Immerse equal volumes of trypsin and milk, stored in different test tubes, in a water bath for 5 mins
Mix together and immediately start timing, record the time taken for the milk to be completely hydrolysed
Test at least 5 temps with at least 3 repeats

Question 4

How is rate of reaction calculated from time

Answer 4

Rate of reaction = 1/time

Question 5

What is the risk associated with handling enzymes

Answer 5

Students may have allergic reactions to enzymes so avoid contact with skin