Coupures Chimiques Flashcards

1
Q

Hydrolyse acide (HCL 6N)

A

Rupture de toutes les liaisons peptidiques

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2
Q

Hydrolyse basique (NaOH 6M)

A

Rupture de toutes les liaisons peptidiques

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3
Q

Méthode de Sanger

A

Identification du N-term

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4
Q

Chlorure de Dabsyl et Dansyl

A

Identification du N-term

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5
Q

Méthode d’Edman

A

Identification du N-term

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6
Q

Mercaptoéthanol, DTT, KCN, Urée

A

Coupent ponts disulfures

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7
Q

Bromure de cyanogène

A

Coupe après Methionine

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8
Q

O-Iodobenzoate

A

Coupe après Tryptophane

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9
Q

Hydroxylamine

A

Coupe liaison Asparagine-Glycine

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10
Q

2-Nitro-5-Thiocyanobenzoate

A

Coupe avant Cystéine

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